Contents: Recombinant Human L-10 Single-Use ELISA RSG Standard
Catalog Number: 39-8109
Formulation: Sterile liquid; phosphate buffered saline, pH 7.2, 150 mM NaCl, with 1.0% BSA. 0.22 µm filtered.
Storage Conditions: The frozen cytokine standard is already aliquoted at 20 µl per vial. Upon receipt, frozen cytokine standard should be immediately stored at -80°C; stable for at least 12 months. After thawing, quick-spin vial prior to opening. Do not re-aliquot into smaller fractions. These are single use vials. Use one time and discard.
Handling Conditions: For best recovery, always quick-spin vial prior to opening. For dilution of current stock, use ELISA Assay Diluent.
Source: E. coli expressed amino acids Ser 19-Asn 178 of mature human IL-10 (accession # NM_000572).
Purity: Greater than 98%, as determined by SDS-PAGE
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Description
Human IL-10 is a ~18 kDa factor also known as cytokine synthesis inhibitory factor (CSIF). Th2 T cells, B1 B cells and keratinocytes are the major cell subsets that produce IL-10. IL-10 inhibits synthesis of Th1 cytokines and proliferation of T cells and acts as a costimulatory signal for mast cells, developing thymocytes and Th2 response.
Applications Reported
For research use only, not for diagnostic or therapeutic use. Recombinant human IL-10 is biologically active. The intended use of the Recombinant Human IL-10 Single-Use ELISA RSG Standard is as a standard for human IL-10 Ready-SET-Go! ELISA and human IL-10 matched antibody pair ELISA. Typically the standard is run in a series of 7 doubling dilutions from the ‘top’ standard described below.
Instructions for use:
For Ready-SET-Go! ELISA Set (TMB substrate). Dilution: Add 6 ul of standard solution to 20 ml of assay diluent to prepare the top standard solution (300 pg/ml).
For matched antibody pair ELISA (ABTS or Super AquaBlue substrate). Dilution: Add 10 ul of standard solution to 10 ml of assay diluent to prepare the top standard solution (1000 pg/ml).
Note: These are single-use vials. Use one time and discard. Dilute as suggested above, not difficult-to-measure fractions of the suggested volumes (e.g., not 0.5 ul in 1.0 ml) or recovery may not be accurate. Use within 6 hours of dilution to top standard concentration.
Applications Tested
This cytokine standard has been calibrated, and subsequently tested by sandwich ELISA using the human IL-10 ELISA pair (JES3-9D7/JES3-12G8).
