Product Information

Contents: Recombinant Mouse IL-10 Single-Use ELISA RSG Standard
Catalog Number: 39-8101
Sizes: 4 pack
Formulation: Phosphate buffered saline, pH 7.2, 150 mM NaCl, with 1.0% BSA, 0.09% NaN₃. 0.22 µm filtered.
Storage Conditions: The frozen cytokine standard is already aliquoted at 20 µl per vial. Upon receipt, frozen cytokine standard should be immediately stored at -80°C; stable for at least 12 months. After thawing, quick-spin vial prior to opening. Do not re-aliquot into smaller fractions. These are single use vials. Use one time and discard.
Handling Conditions: For best recovery, always quick-spin vial prior to opening. For dilution of current stock, use ELISA Assay Diluent.
Source: E. coli expressed amino acids ser 19-ser 178 of mature mouse IL-10 (accession # NM_010548) with a cys149tyr substitution (Moore, K. et al., Science 248:1230-1234).
Purity: Greater than 98%, as determined by SDS-PAGE

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-8101	Recombinant Mouse IL-10 (Interleukin-10, IL10)	N/A	N/A	BA ELISA
34-8101	Carrier-Free Recombinant Mouse IL-10 (Interleukin-10, IL10)	N/A	N/A	BA ELISA
39-8101	Single-Use ELISA RSG Standard Recombinant Mouse IL-10 (Interleukin-10, IL10)	N/A	N/A	ELISA

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Description

Mouse IL-10 is an ~18 kDa cytokine, also known as Cytokine Synthesis Inhibitory Factor (CSIF). In the mouse, Th2 cells, B1 cells and keratinocytes are the major cell subsets that produce IL-10. IL-10 inhibits synthesis of Th1 cytokines and proliferation of T cells, and acts as a costimulatory signal for mast cells, developing thymocytes and the Th2 response.

Applications Reported

For research use only, not for diagnostic or therapeutic use. Recombinant mouse IL-10 is biologically active. The intended use of the Recombinant Mouse IL-10 Single-Use ELISA RSG Standard is as a standard for mouse IL-10 Ready-SET-Go! ELISA and mouse IL-10 matched antibody pair ELISA. Typically the standard is run in a series of 7 doubling dilutions from the ‘top’ standard described below.

Instructions for use:
For Ready-SET-Go! ELISA Set (TMB substrate). Dilution: Add 20 ul of standard solution to 5 ml of assay diluent to prepare the top standard solution (4000 pg/ml).
For matched antibody pair ELISA (ABTS or Super AquaBlue substrate). Dilution: Add 20 ul of standard solution to 5 ml of assay diluent to prepare the top standard solution (4000 pg/ml).

Note: These are single-use vials. Use one time and discard. Dilute as suggested above, not difficult-to-measure fractions of the suggested volumes (e.g., not 0.5 ul in 1.0 ml) or recovery may not be accurate. Use within 6 hours of dilution to top standard concentration.

Applications Tested

This cytokine standard has been calibrated, and subsequently tested by sandwich ELISA using the mouse IL-10 ELISA pair (JES5-16E3/JES5-2A5).