Contents: Recombinant Mouse IL-10 Single-Use ELISA RSG Standard
Catalog Number: 39-8101
Formulation: Sterile liquid; phosphate buffered saline, pH 7.2, 150 mM NaCl, with 1.0% BSA. 0.22 µm filtered.
Storage Conditions: The frozen cytokine standard is already aliquoted at 20 µl per vial. Upon receipt, frozen cytokine standard should be immediately stored at -80°C; stable for at least 12 months. After thawing, quick-spin vial prior to opening. Do not re-aliquot into smaller fractions. These are single use vials. Use one time and discard.
Handling Conditions: For best recovery, always quick-spin vial prior to opening. For dilution of current stock, use ELISA Assay Diluent.
Source: E. coli expressed amino acids ser 19-ser 178 of mature mouse IL-10 (accession # NM_010548) with a cys149tyr substitution (Moore, K. et al., Science 248:1230-1234).
Purity: Greater than 98%, as determined by SDS-PAGE
| Prices for This Product* |
| Cat. No. |
Size |
Price |
Qty |
Action |
*International customers: Please contact your distributor for region specific pricing.
|
|
|
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
Mouse IL-10 is an ~18 kDa cytokine, also known as Cytokine Synthesis Inhibitory Factor (CSIF). In the mouse, Th2 cells, B1 cells and keratinocytes are the major cell subsets that produce IL-10. IL-10 inhibits synthesis of Th1 cytokines and proliferation of T cells, and acts as a costimulatory signal for mast cells, developing thymocytes and the Th2 response.
Applications Reported
For research use only, not for diagnostic or therapeutic use. Recombinant mouse IL-10 is biologically active. The intended use of the Recombinant Mouse IL-10 Single-Use ELISA RSG Standard is as a standard for mouse IL-10 Ready-SET-Go! ELISA and mouse IL-10 matched antibody pair ELISA. Typically the standard is run in a series of 7 doubling dilutions from the ‘top’ standard described below.
Instructions for use:
For Ready-SET-Go! ELISA Set (TMB substrate). Dilution: Add 20 ul of standard solution to 5 ml of assay diluent to prepare the top standard solution (4000 pg/ml).
For matched antibody pair ELISA (ABTS or Super AquaBlue substrate). Dilution: Add 20 ul of standard solution to 5 ml of assay diluent to prepare the top standard solution (4000 pg/ml).
Note: These are single-use vials. Use one time and discard. Dilute as suggested above, not difficult-to-measure fractions of the suggested volumes (e.g., not 0.5 ul in 1.0 ml) or recovery may not be accurate. Use within 6 hours of dilution to top standard concentration.
Applications Tested
This cytokine standard has been calibrated, and subsequently tested by sandwich ELISA using the mouse IL-10 ELISA pair (JES5-16E3/JES5-2A5).
