Contents: Recombinant Human IL-5 Single-Use ELISA RSG Standard
Catalog Number: 39-8059
Formulation: Sterile liquid; phosphate buffered saline, pH 7.2, 150 mM NaCl, with 1.0% BSA. 0.22 µm filtered.
Storage Conditions: The frozen cytokine standard is already aliquoted at 20 µl per vial. Upon receipt, frozen cytokine standard should be immediately stored at -80°C; stable for at least 12 months. After thawing, quick-spin vial prior to opening. Do not re-aliquot into smaller fractions. These are single use vials. Use one time and discard.
Handling Conditions: For best recovery, always quick-spin vial prior to opening. For dilution of current stock, always include carrier protein (1% BSA or 10% FBS) in the buffered saline diluent.
Source: E. coli
Purity: Greater than 98%, as determined by SDS-PAGE
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Description
Human IL-5 (also known as BCGF and BCDF) is a ~40 kDa factor produced mainly by T cells. IL-5 is present as an N-glycosylated homodimer and is a hematopoietic growth factor promoting proliferation, activation and differentiation of eosinophil granulocytes. In the human, IL-5 also supports generation of cytotoxic T cells. In the mouse, IL-5 acts on B cells to induce proliferation and secretion of IgM and IgA from endotoxin activated B cells.
Applications Reported
For research use only, not for diagnostic or therapeutic use. Recombinant human IL-5 is biologically active. The intended use of the Recombinant Human IL-5 Single-Use ELISA RSG Standard is as a standard for human IL-5 Ready-SET-Go! ELISA and human IL-5 matched antibody pair ELISA. Typically the standard is run in a series of 7 doubling dilutions from the ‘top’ standard described below.
Instructions for use:
For Ready-SET-Go! ELISA Set (TMB substrate). Dilution: Add 5 ul of standard solution to 10 ml of assay diluent to prepare the top standard solution (500 pg/ml).
For matched antibody pair ELISA (ABTS or Super AquaBlue substrate). Dilution: Add 5 ul of standard solution to 10 ml of assay diluent to prepare the top standard solution (500 pg/ml).
Note: These are single-use vials. Use one time and discard. Dilute as suggested above, not difficult-to-measure fractions of the suggested volumes (e.g., not 0.5 ul in 1.0 ml) or recovery may not be accurate. Use within 6 hours of dilution to top standard concentration.
Applications Tested
This cytokine standard has been calibrated, and subsequently tested by sandwich ELISA using the human IL-5 ELISA pair (TRFK5/JES1-5A10).
