Product Information

Contents: Recombinant Human IL-4 Single-Use ELISA RSG Standard
Catalog Number: 39-8049
Sizes: 4 pack
Formulation: Sterile liquid; phosphate buffered saline, pH 7.2, 150 mM NaCl, with 1.0% BSA. 0.22 µm filtered.
Storage Conditions: The frozen cytokine standard is already aliquoted at 20 µl per vial. Upon receipt, frozen cytokine standard should be immediately stored at -80°C; stable for at least 12 months. After thawing, quick-spin vial prior to opening. Do not re-aliquot into smaller fractions. These are single use vials. Use one time and discard.
Handling Conditions: For best recovery, always quick-spin vial prior to opening. For dilution of current stock, always include carrier protein (1% BSA or 10% FBS) in the buffered saline diluent.
Source: E. coli expressed amino acids His 25-Ser 153 of mature human IL-4 (accession # AF 395008).
Purity: Greater than 98%, as determined by SDS-PAGE

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-8049	Recombinant Human IL-4 (Interleukin-4, IL4)	N/A	N/A	BA ELISA
34-8049	Carrier-Free Recombinant Human IL-4 (Interleukin-4, IL4)	N/A	N/A	BA ELISA
39-8049	Single-Use ELISA RSG Standard Recombinant Human IL-4 (Interleukin-4, IL4)	N/A	N/A	ELISA

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

Human IL-4 is a ~15-19 kDa factor also known as BCDF, BCGF-1 and BSF-1. IL-4 is secreted by activated Th2 and natural killer T cells and to a lesser extent by Th1 and mast cells. IL-4 is species-specific and promotes proliferation and differentiation of B cells. IL-4 in conjunction with other signals such as CD40 induces Ig isotype switching of B cells and upregulation of surface antigens including MHC class II and CD23.

Applications Reported

For research use only, not for diagnostic or therapeutic use. Recombinant human IL-4 is biologically active. The intended use of the Recombinant Human IL-4 Single-Use ELISA RSG Standard is as a standard for human IL-4 Ready-SET-Go! ELISA and human IL-4 matched antibody pair ELISA. Typically the standard is run in a series of 7 doubling dilutions from the ‘top’ standard described below.

Instructions for use:
For Ready-SET-Go! ELISA Set (TMB substrate). Dilution: Add 4 ul of standard solution to 20 ml of assay diluent to prepare the top standard solution (200 pg/ml).
For matched antibody pair ELISA (ABTS or Super AquaBlue substrate). Dilution: Add 5 ul of standard solution to 10 ml of assay diluent to prepare the top standard solution (500 pg/ml).

Note: These are single-use vials. Use one time and discard. Dilute as suggested above, not difficult-to-measure fractions of the suggested volumes (e.g., not 0.5 ul in 1.0 ml) or recovery may not be accurate. Use within 6 hours of dilution to top standard concentration.

Applications Tested

This cytokine standard has been calibrated, and subsequently tested by sandwich ELISA using the human IL-4 ELISA pair (8D4-8/MP4-25D2).