Product Information

Contents: Recombinant Human IL-1b Single-Use ELISA RSG Standard
Catalog Number: 39-8018
Sizes: 4 pack
Formulation: Sterile liquid; phosphate buffered saline, pH 7.2, 150 mM NaCl, with 1.0% BSA. 0.22 µm filtered.
Storage Conditions: The frozen cytokine standard is already aliquoted at 20 µl per vial. Upon receipt, frozen cytokine standard should be immediately stored at -80°C; stable for at least 12 months. After thawing, quick-spin vial prior to opening. Do not re-aliquot into smaller fractions. These are single use vials. Use one time and discard.
Handling Conditions: For best recovery, always quick-spin vial prior to opening. For dilution of current stock, use ELISA Assay Diluent.
Source: E. coli expressed amino acids Ala 117-Ser 269 of human IL-1b (accession # NM_000576).
Molecular Mass: The protein is not methionylated at the N-terminal and has a predicted molecular mass of 17,377. The DTT reduced and non-reduced proteins migrate as 18 kDa polypeptides on SDS-PAGE.
Purity: Greater than 98%, as determined by SDS-PAGE

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-8018	Recombinant Human IL-1b (Interleukin-1 beta, IL1b)	N/A	N/A	BA ELISA
34-8018	Carrier-Free Recombinant Human IL-1b (Interleukin-1 beta, IL1b)	N/A	N/A	BA ELISA
39-8018	Single-Use ELISA RSG Standard Recombinant Human IL-1b (Interleukin-1 beta, IL1b)	N/A	N/A	ELISA std

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

Human IL-1β, also called lymphocyte activating factor (LAF), endogenous pyrogen (EP), leucocyte endogenous mediator (LEM), mononuclear cell factor (MCF), is produced by a wide variety of cells, including macrophages, dendritic cells, T cells and B cells. IL-1β is mostly released and has only 20% amino acid homology with IL-1α. The immune regulatory role of IL-1β is exerted on a wide range of cells including lymphocytes, epithelial cells and fibroblasts. In vivo, it induces hypotension, fever, and acute phase response.

Applications Reported

For research use only, not for diagnostic or therapeutic use. Recombinant human IL-1β is biologically active. The intended use of the Recombinant Human IL-1b Single-Use ELISA RSG Standard is as a standard for human IL-1ß Ready-SET-Go! ELISA and human IL-1ß matched antibody pair ELISA. Typically the standard is run in a series of 7 doubling dilutions from the ‘top’ standard described below.

Instructions for use:
For Ready-SET-Go! ELISA Set (TMB substrate). Dilution: Add 5 ul of standard solution to 10 ml of assay diluent to prepare the top standard solution (500 pg/ml).
For matched antibody pair ELISA (ABTS or Super AquaBlue substrate). Dilution: Add 10 ul of standard solution to 10 ml of assay diluent to prepare the top standard solution (1000 pg/ml).

Note: These are single-use vials. Use one time and discard. Dilute as suggested above, not difficult-to-measure fractions of the suggested volumes (e.g., not 0.5 ul in 1.0 ml) or recovery may not be accurate. Use within 6 hours of dilution to top standard concentration.

Applications Tested

This cytokine standard has been calibrated and tested by sandwich ELISA using the human IL-1ß ELISA pair (CRM56/CRM57).

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