Product Information

Contents: Recombinant Mouse IL-1β Single-Use ELISA RSG Standard
Catalog Number: 39-8012
Sizes: 4 pack
Formulation: Phosphate buffered saline, pH 7.2, 150 mM NaCl, with 1.0% BSA, 0.09% NaN₃. 0.22 µm filtered.
Storage Conditions: The frozen cytokine standard is already aliquoted at 20 µl per vial. Upon receipt, frozen cytokine standard should be immediately stored at -80°C; stable for at least 12 months. After thawing, quick-spin vial prior to opening. Do not re-aliquot into smaller fractions. These are single use vials. Use one time and discard.
Handling Conditions: For best recovery, always quick-spin vial prior to opening. For dilution of current stock, use ELISA Assay Diluent.
Source: E. coli expressed amino acids Val 118-Ser 269 of mouse IL-1β (accession # P10749).
Purity: Greater than 98%, as determined by SDS-PAGE

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-8012	Recombinant Mouse IL-1b (Interleukin-1 beta, IL1b)	N/A	N/A	BA ELISA
34-8012	Carrier-Free Recombinant Mouse IL-1b (Interleukin-1 beta, IL1b)	N/A	N/A	BA ELISA
39-8012	Single-Use ELISA RSG Standard Recombinant Mouse IL-1b (Interleukin-1 beta, IL1b)	N/A	N/A	ELISA

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Description

Mouse IL-1β, also called lymphocyte activating factor (LAF), endogenous pyrogen (EP), leucocyte endogenous mediator (LEM), mononuclear cell factor (MCF), is a ~17 kDa factor produced by a wide variety of cells, including macrophages, dendritic cells, T and B cells. IL-1β is mostly cell associated with 23% amino acid homology with IL-1α. The immune regulatory role of IL-1β is exerted on a wide range of cells including lymphocytes, epithelial cells and fibroblasts. In vivo, it induces hypotension, fever, and acute phase response.

Applications Reported

For research use only, not for diagnostic or therapeutic use. Recombinant mouse IL-1β is biologically active. The intended use of the Recombinant Mouse IL-1β Single-Use ELISA RSG Standard is as a standard for mouse IL-1β Ready-SET-Go! ELISA and mouse IL-1β matched antibody pair ELISA. Typically the standard is run in a series of 7 doubling dilutions from the ‘top’ standard described below.

Instructions for use:
For Ready-SET-Go! ELISA Set (TMB substrate). Dilution: Add 10 ul of standard solution to 10 ml of assay diluent to prepare the top standard solution (1000 pg/ml).
For matched antibody pair ELISA (ABTS or Super AquaBlue substrate). Dilution: Add 20 ul of standard solution to 10 ml of assay diluent to prepare the top standard solution (2000 pg/ml).

Note: These are single-use vials. Use one time and discard. Dilute as suggested above, not difficult-to-measure fractions of the suggested volumes (e.g., not 0.5 ul in 1.0 ml) or recovery may not be accurate. Use within 6 hours of dilution to top standard concentration.