Contents: Recombinant Mouse IL-1α Single-Use ELISA RSG Standard
Catalog Number: 39-8011
Formulation: Sterile liquid; phosphate buffered saline, pH 7.2, 150 mM NaCl, with 1.0% BSA. 0.22 µm filtered.
Storage Conditions: The frozen cytokine standard is already aliquoted at 20 µl per vial. Upon receipt, frozen cytokine standard should be immediately stored at -80°C; stable for at least 12 months. After thawing, quick-spin vial prior to opening. Do not re-aliquot into smaller fractions. These are single use vials. Use one time and discard.
Handling Conditions: For best recovery, always quick-spin vial prior to opening. For dilution of current stock, always include carrier protein (1% BSA or 10% FBS) in the buffered saline diluent.
Source: E. coli expressed amino acids Ser 115-Ser 270 of mouse IL-1α (accession # BC003727).
Purity: Greater than 98%, as determined by SDS-PAGE
Endotoxin Level: Less than 0.01 ng/ug cytokine as determined by the LAL assay.
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Description
Mouse IL-1α, also called Lymphocyte Activating Factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), and Mononuclear Cell Factor (MCF), is an ~17 kDa factor produced by a wide variety of cells, including macrophages, dendritic cells, T and B cells. IL-1α is mostly cell-associated, with 23% amino acid homology with IL-1β. The immune regulatory role of IL-1α is exerted on a wide range of cells including lymphocytes, epithelial cells and fibroblasts.
In vivo, it induces hypotension, fever, and acute phase response.
Applications Reported
For research use only, not for diagnostic or therapeutic use. Recombinant mouse IL-1α is biologically active. The intended use of the Recombinant Mouse IL-1α Single-Use ELISA RSG Standard is as a standard for mouse IL-1α matched antibody pair ELISA. Typically the standard is run in a series of 7 doubling dilutions from the ‘top’ standard described below.
For matched antibody pair ELISA (ABTS or Super AquaBlue substrate). Dilution: Add 10 ul of standard solution to 10 ml of assay diluent to prepare the top standard solution (1000 pg/ml).
Note: These are single-use vials. Use one time and discard. Dilute as suggested above, not difficult-to-measure fractions of the suggested volumes (e.g., not 0.5 ul in 1.0 ml) or recovery may not be accurate. Use within 6 hours of dilution to top standard concentration.
Applications Tested
This cytokine standard has been calibrated, and subsequently tested by sandwich ELISA using the mouse IL-1α ELISA pair (ALF-161/polyclonal antibody).
