mouse IFNg (Interferon-gamma, IFN-g)

Contents: In Vivo Cytokine Capture Assay (IVCCA) In Vivo Capture Antibody / Functional Grade Biotin anti-mouse IFN-γ
Catalog Number: 36-7312
Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, No NaN3. Endotoxin level is less than 0.05 ng/ug antibody, as determined by the LAL assay.
Storage Conditions: Store at 4°C. Do not freeze. Keep contents sterile.
Clone: R4-6A2
Isotype: Rat IgG1, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
36-7312-85	500 ug	$320

*International customers: Please contact your distributor for region specific pricing.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
13-7312	Biotin anti-mouse IFNg (Interferon-gamma, IFN-g)	N/A	N/A	ELISA det ELISPOT
14-7312	Affinity Purified anti-mouse IFNg (Interferon-gamma, IFN-g)	N/A	N/A	NU
16-7312	Functional Grade* Purified anti-mouse IFNg (Interferon-gamma, IFN-g)	N/A	N/A	NU
36-7312	In Vivo Cytokine Capture Assay (IVCCA) In Vivo Capture Antibody / Functional Grade* Biotin anti-mouse IFNg (Interferon-gamma, IFN-g)	N/A	N/A	IVCCA
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The R4-6A2 antibody reacts with mouse interferon-γ (IFN-γ ), a homodimeric 15-20 kDa cytokine secreted by Th1 cells. The R4-6A2 antibody is a neutralizing antibody. IFN-γ is an ~20 kDa factor produced by activated T, B and NK cells and is an anti-viral and anti-parasitic cytokine. IFN-γ in synergy with other cytokines, such as TNF-α, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN-γ are exerted on a wide range of cell types expressing the high affinity receptors for IFN-γ. Glycosylation of IFN-γ does not affect its biological activity.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The Functional Grade Biotin anti-mouse IFNg (R4-6A2) has been reported for use as the in vivo capture antibody for the In Vivo Cytokine Capture Assay (IVCCA). The IVCCA facilitates measurement of cytokines in serum by increasing their in vivo half lives dramatically. This increases the sensitivity of measurement of in vivo cytokine production 30- to 1000- fold.

For detailed methodology in this application, please refer to this reference: Finkelman, F., S. Morris, T. Orekhova, and D. Sehy. 2003. The In Vivo Cytokine Capture Assay for measurement of cytokine production in the mouse. In Current Protocols in Immunology. Unit 6.28. J. Coligan, A. Kruisbeek, D. Margulies, E. Shevach, and W. Strober, eds. John Wiley and Sons, New York

Applications Tested

The Functional Grade Biotin R4-6A2 antibody has been tested by LAL assay to ensure it is free of endotoxin, and has been tested as the detection antibody in a sandwich ELISA for analysis of mouse IFNg (Interferon-gamma, IFN-g) in combination with the purified AN-18 (14-7313) antibody for capture and recombinant mouse IFNg (14-8311) as the standard.

A suitable range of concentrations of the FG Biotin R4-6A2 antibody for ELISA detection is 0.5-2 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 200 pg/ml - 1.6 pg/ml (AN-18 assay) should be included in each ELISA plate.

References

Spitalny, G. L. and E. A. Havell. 1984. Monoclonal antibody to murine gamma interferon inhibits lymphokine-induced antiviral and macrophage tumoricidal activities. J Exp Med. 159: 1560-5.
Abrams, J. 1995. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. In Current Protocols in Immunology. A. Kruisbeek eds. Wiley-Interscience, New York. Unit 6.20.1.
Finkelman, F., S. Morris, T. Orekhova, and D. Sehy. 2003. The In Vivo Cytokine Capture Assay for measurement of cytokine production in the mouse. In Current Protocols in Immunology. Unit 6.28. J. Coligan, A. Kruisbeek, D. Margulies, E. Shevach, and W. Strober, eds. John Wiley and Sons, New York.
Finkelman, F.D., and S.C. Morris. 1999. Development of an assay to measure in vivo cytokine production in the mouse. Int. Immunology. 11: 1811-1818.

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