mouse IL-10 (Interleukin-10)

Contents: In Vivo Cytokine Capture Assay (IVCCA) In Vivo Capture Antibody / Functional Grade Biotin anti-mouse IL-10
Catalog Number: 36-7101
Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, No NaN3. Endotoxin level is less than 0.05 ng/ug antibody, as determined by the LAL assay.
Storage Conditions: Store at 4°C. Do not freeze. Keep contents sterile.
Clone: JES5-16E3
Isotype: Rat IgG2b, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
36-7101-85	500 ug	$320

*International customers: Please contact your distributor for region specific pricing.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-7101	FITC anti-mouse IL-10 (Interleukin-10, IL10)	488	518	IC Flow
12-7101	PE anti-mouse IL-10 (Interleukin-10, IL10)	488	575	IC Flow
14-7101	Affinity Purified anti-mouse IL-10 (Interleukin-10, IL10)	N/A	N/A	ELISA cap ELISPOT cap
16-7101	Functional Grade* Purified anti-mouse IL-10 (Interleukin-10, IL10)	N/A	N/A	ELISA cap ELISPOT cap NU
17-7101	APC anti-mouse IL-10 (Interleukin-10, IL10)	633	660	IC Flow
36-7101	In Vivo Cytokine Capture Assay (IVCCA) In Vivo Capture Antibody / Functional Grade* Biotin anti-mouse IL-10 (Interleukin-10, IL10)	N/A	N/A	IVCCA
45-7101	PerCP-Cy5.5 anti-mouse IL-10 (Interleukin-10)	488	690	IC Flow
51-7101	Alexa Fluor® 647 anti-mouse IL-10 (Interleukin-10, IL10)	633	668	IC Flow
53-7101	Alexa Fluor® 488 anti-mouse IL-10 (Interleukin-10, IL10)	488	519	IC Flow
56-7101	Alexa Fluor® 700 anti-mouse IL-10 (Interleukin-10)	633	723	IC Flow
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The JES5-16E3 antibody reacts with mouse interleukin-10 (IL-10). Mouse IL-10 is an ~18 kDa factor also known as Cytokine Synthesis Inhibitory Factor (CSIF). In the mouse, Th2 cells, B1 cells, macrophages, and keratinocytes are the major cell subsets that produce IL-10. IL-10 inhibits synthesis of Th1 cytokines and proliferation of T cells, and acts as a costimulatory signal for mast cells, developing thymocytes and the Th2 response.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The Functional Grade Biotin anti-mouse IL-10 antibody (clone JES5-16E3) has been reported for use as the in vivo capture antibody for the In Vivo Cytokine Capture Assay (IVCCA). The IVCCA facilitates measurement of cytokines in serum by increasing their in vivo half lives dramatically. This increases the sensitivity of measurement of in vivo cytokine production 30- to 1000- fold.

For detailed methodology in this application, please refer to this reference: Finkelman, F., S. Morris, T. Orekhova, and D. Sehy. 2003. The In Vivo Cytokine Capture Assay for measurement of cytokine production in the mouse. In Current Protocols in Immunology. Unit 6.28. J. Coligan, A. Kruisbeek, D. Margulies, E. Shevach, and W. Strober, eds. John Wiley and Sons, New York

Applications Tested

The Functional Grade Biotin JES5-16E3 antibody has been tested by LAL assay to ensure it is free of endotoxin, and has been tested as the detection antibody in a sandwich ELISA for analysis of mouse IL-10 in combination with the purified JES5-2A5 (14-7102) antibody for capture and recombinant mouse IL-10 (14-8101) as the standard.

A suitable range of concentrations of the FG Biotin JES5-16E3 antibody for ELISA detection is 0.5-2 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 4000 pg/ml - 30 pg/ml should be included in each ELISA plate.

References

Sander, B., I. Hoiden, et al. 1993. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining. J Immunol Meth. 1662: 201-14.
Abrams, J. 1995. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. In Current Protocols in Immunology. A. Kruisbeek eds. Wiley-Interscience, New York. Unit 6.20.1.
Finkelman, F., S. Morris, T. Orekhova, and D. Sehy. 2003. The In Vivo Cytokine Capture Assay for measurement of cytokine production in the mouse. In Current Protocols in Immunology. Unit 6.28. J. Coligan, A. Kruisbeek, D. Margulies, E. Shevach, and W. Strober, eds. John Wiley and Sons, New York.
Finkelman, F.D., and S.C. Morris. 1999. Development of an assay to measure in vivo cytokine production in the mouse. Int. Immunology. 11: 1811-1818.

Related Products

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Cat. 14-7102    Affinity Purified anti-mouse IL-10 (Interleukin-10, IL10) (clone JES5-2A5)
Cat. 14-8101    Recombinant Mouse IL-10 (Interleukin-10, IL10)