Product Information

Contents: In Vivo Cytokine Capture Assay (IVCCA) In Vivo Capture Antibody / Functional Grade Biotin anti-mouse IL-4 (Interleukin-4)
Catalog Number: 36-7043
Sizes: 500 ug
Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, No NaN3. Endotoxin level is less than 0.05 ng/ug antibody, as determined by the LAL assay.
Storage Conditions: Store at 4°C. Do not freeze. Keep contents sterile.
Clone: BVD4-1D11
Isotype: Rat IgG2b

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
36-7043	DISCONTINUED - In Vivo Cytokine Capture Assay (IVCCA) In Vivo Capture Antibody / Functional Grade* Biotin anti-mouse IL-4 (Interleukin-4, IL4)	N/A	N/A	IVCCA
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The BVD4-1D11 antibody reacts with mouse interleukin-4 (IL-4), a 14 kDa cytokine secreted by Th2 cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The Functional Grade Biotin anti-mouse IL-4 antibody (clone BVD4-1D11) has been reported for use as the in vivo capture antibody for the In Vivo Cytokine Capture Assay (IVCCA). The IVCCA facilitates measurement of cytokines in serum by increasing their in vivo half lives dramatically. This increases the sensitivity of measurement of in vivo cytokine production 30- to 1000- fold.

For detailed methodology in this application, please refer to this reference: Finkelman, F., S. Morris, T. Orekhova, and D. Sehy. 2003. The In Vivo Cytokine Capture Assay for measurement of cytokine production in the mouse. In Current Protocols in Immunology. Unit 6.28. J. Coligan, A. Kruisbeek, D. Margulies, E. Shevach, and W. Strober, eds. John Wiley and Sons, New York

Applications Tested

The Functional Grade Biotin BVD4-1D11 antibody has been tested by LAL assay to ensure it is free of endotoxin, and has been tested as the detection antibody in a sandwich ELISA for analysis of mouse IL-4 in combination with the purified BVD6-24G2 (14-7042) antibody for capture and recombinant mouse IL-4 (14-8041) as the standard.

A suitable range of concentrations of the FG Biotin BVD4-1D11 antibody for ELISA detection is 0.5-2 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 500 pg/ml - 4.0 pg/ml should be included in each ELISA plate.

References

Abrams, J. 1995. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. In Current Protocols in Immunology. A. Kruisbeek eds. Wiley-Interscience, New York. Unit 6.20.1.
Finkelman, F., S. Morris, T. Orekhova, and D. Sehy. 2003. The In Vivo Cytokine Capture Assay for measurement of cytokine production in the mouse. In Current Protocols in Immunology. Unit 6.28. J. Coligan, A. Kruisbeek, D. Margulies, E. Shevach, and W. Strober, eds. John Wiley and Sons, New York.
Finkelman, F.D., and S.C. Morris. 1999. Development of an assay to measure in vivo cytokine production in the mouse. Int. Immunology. 11: 1811-1818.

Related Products

Cat. 36-4031    In Vivo Cytokine Capture Assay (IVCCA) In Vivo Capture Antibody – Control / Functional Grade Biotin Rat IgG2b Isotype Control
Cat. 24-5093    Polyclonal anti-mouse IgD antiserum (activating) (clone Polyclonal)
Cat. 14-7042    Affinity Purified anti-mouse IL-4 (Interleukin-4, IL4) (clone BVD6-24G2)
Cat. 14-8041    Recombinant Mouse IL-4 (Interleukin-4, IL4)