mouse IL-2 (Interleukin-2)

Contents: In Vivo Cytokine Capture Assay (IVCCA) In Vivo Capture Antibody / Functional Grade Biotin anti-mouse IL-2 (Interleukin-2)
Catalog Number: 36-7021
Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, No NaN3. Endotoxin level is less than 0.05 ng/ug antibody, as determined by the LAL assay.
Storage Conditions: Store at 4°C. Do not freeze. Keep contents sterile.
Clone: JES6-5H4
Isotype: Rat IgG2b, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
36-7021-85	500 ug	$320

*International customers: Please contact your distributor for region specific pricing.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-7021	FITC anti-mouse IL-2 (Interleukin-2, IL2)	488	518	IC Flow
12-7021	PE anti-mouse IL-2 (Interleukin-2, IL2)	488	575	IC Flow
13-7021	Biotin anti-mouse IL-2 (Interleukin-2, IL2)	N/A	N/A	ELISA det ELISPOT det IP
14-7021	Affinity Purified anti-mouse IL-2 (Interleukin-2, IL2)	N/A	N/A	IC Flow IP
16-7021	Functional Grade* Purified anti-mouse IL-2 (Interleukin-2, IL2)	N/A	N/A	IC Flow
17-7021	APC anti-mouse IL-2 (Interleukin-2, IL2)	633	660	IC Flow
36-7021	In Vivo Cytokine Capture Assay (IVCCA) In Vivo Capture Antibody / Functional Grade* Biotin anti-mouse IL-2 (Interleukin-2, IL2)	N/A	N/A	IVCCA
45-7021	PerCP-Cy5.5 anti-mouse IL-2 (Interleukin-2)	488	690	IC Flow
51-7021	Alexa Fluor® 647 anti-mouse IL-2 (Interleukin-2, IL2)	633	668	IC Flow
53-7021	Alexa Fluor® 488 anti-mouse IL-2 (Interleukin-2, IL2)	488	519	IC Flow
57-7021	Pacific Blue® anti-mouse IL-2 (Interleukin-2, IL2)	402	421	IC Flow
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The JES6-5H4 antibody reacts with mouse interleukin-2 (IL-2), a 17 kDa T cell growth factor and a major immunoregulatory cytokine.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The Functional Grade Biotin anti-mouse IL-2 antibody (clone JES6-5H4) has been reported for use as the in vivo capture antibody for the In Vivo Cytokine Capture Assay (IVCCA). The IVCCA facilitates measurement of cytokines in serum by increasing their in vivo half lives dramatically. This increases the sensitivity of measurement of in vivo cytokine production 30- to 1000- fold.

For detailed methodology in this application, please refer to this reference: Finkelman, F., S. Morris, T. Orekhova, and D. Sehy. 2003. The In Vivo Cytokine Capture Assay for measurement of cytokine production in the mouse. In Current Protocols in Immunology. Unit 6.28. J. Coligan, A. Kruisbeek, D. Margulies, E. Shevach, and W. Strober, eds. John Wiley and Sons, New York

Applications Tested

The Functional Grade Biotin JES6-5H4 antibody has been tested by LAL assay to ensure it is free of endotoxin, and has been tested as the detection antibody in a sandwich ELISA for analysis of mouse IL-2 in combination with the purified JES6-1A12 (14-7022) antibody for capture and recombinant mouse IL-2 (14-8021) as the standard.

A suitable range of concentrations of the FG Biotin JES6-5H4 antibody for ELISA detection is 0.5-2 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 500 pg/ml - 4.0 pg/ml should be included in each ELISA plate.

References

Abrams, J. S., M. G. Roncarolo, et al. (1992). Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples. Immunol Rev. 127: 5-24.
Sander, B., I. Hoiden, et al. 1993. Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining. J Immunol Meth. 166(2): 201-14.
Finkelman, F., S. Morris, T. Orekhova, and D. Sehy. 2003. The In Vivo Cytokine Capture Assay for measurement of cytokine production in the mouse. In Current Protocols in Immunology. Unit 6.28. J. Coligan, A. Kruisbeek, D. Margulies, E. Shevach, and W. Strober, eds. John Wiley and Sons, New York.
Finkelman, F.D., and S.C. Morris. 1999. Development of an assay to measure in vivo cytokine production in the mouse. Int. Immunology. 11: 1811-1818.

Related Products

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Cat. 24-5093    Polyclonal anti-mouse IgD antiserum (activating) (clone Polyclonal)
Cat. 14-7022    Affinity Purified anti-mouse IL-2 (Interleukin-2, IL2) (clone JES6-1A12)
Cat. 14-8021    Recombinant Mouse IL-2 (Interleukin-2, IL2)