Contents: Functional Grade Biotin anti-mouse TER-119 (TER119, Erythroid cells, Ly-76)
Catalog Number: 36-5921
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN3
Storage Conditions: Store at 4°C.
Clone: TER-119
Isotype: Rat IgG2b, κ
| Prices for This Product* |
| Cat. No. |
Size |
Price |
Qty |
Action |
*International customers: Please contact your distributor for region specific pricing.
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Staining of BALB/c bone marrow cells with 0.25 μg of PE Rat IgG2b Iso Cntrl (cat. 12-4032) (open histogram) or 0.25 μg of Biotin TER-119 (colored histogram) followed by Streptavidin-PE (cat. 12-4317). Cells in the small scatter population were used for analysis.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 11-5921 |
FITC anti-mouse TER-119 (TER119, Erythroid cells, Ly-76) |
488 |
518 |
FC
|
| 12-5921 |
PE anti-mouse TER-119 (TER119, Erythroid cells, Ly-76) |
488 |
575 |
FC
|
| 13-5921 |
Biotin anti-mouse TER-119 (TER119, Erythroid cells, Ly-76) |
N/A |
N/A |
FC
|
| 14-5921 |
Affinity Purified anti-mouse TER-119 (TER119, Erythroid cells, Ly-76) |
N/A |
N/A |
FC
IHC
IP
WB
|
| 15-5921 |
PE-Cy5 anti-mouse TER-119 (TER119, Erythroid cells, Ly-76) |
488 |
670 |
FC
|
| 16-5921 |
Functional Grade* Purified anti-mouse TER-119 (TER119, Erythroid cells, Ly-76) |
N/A |
N/A |
FC
|
| 17-5921 |
APC anti-mouse TER-119 (TER119, Erythroid cells, Ly-76) |
633 |
660 |
FC
|
| 25-5921 |
PE-Cy7 anti-mouse TER-119 (TER119, Erythroid cells, Ly-76) |
488 |
760 |
FC
|
| 27-5921 |
Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-mouse TER-119 (TER119, Erythroid cells, Ly-76) (APC-Cy7 Equivalent) |
633 |
779 |
FC
|
| 35-5921 |
Phycoerythrin-Cy5.5 (PE-Cy5.5) anti-mouse TER-119 (TER119, Erythroid cells, Ly-76) |
488 |
690 |
FC
|
| 36-5921 |
Functional Grade* Biotin anti-mouse TER-119 (TER119, Erythroid cells, Ly-76) |
N/A |
N/A |
FC
|
| 45-5921 |
PerCP-Cy5.5 anti-mouse TER-119 (TER119, Erythroid cells, Ly-76) |
488 |
690 |
FC
|
| 57-5921 |
Pacific Blue® anti-mouse TER-119 (TER119, Erythroid cells, Ly-76) |
402 |
421 |
FC
|
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The TER-119 monoclonal antibody reacts with mouse erythroid cells from early proerythroblast to mature erythrocyte stages. The TER-119 antigen is present in yolk sac, fetal and newborn liver, but is not expressed by cells carrying BFU-E and CFU-E activities. Several erythroleukemia cell lines tested so far are negative for expression of TER-119 antigen even after dimethylsulfoxide stimulation. Biochemical and molecular analysis of the TER-119 antigen indicate that this molecule is associated with the surface glycophorin A, but is not a typical glycophorin.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This TER-119 antibody has been reported for use in flow cytometric analysis.
Applications Tested
This TER-119 antibody has been tested by flow cytometric analysis of mouse splenocyte and bone marrow cell suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Kina, T., K. Ikuta, et al. (2000). "The monoclonal antibody TER-119 recognizes a molecule associated with glycophorin A and specifically marks the late stages of murine erythroid lineage." Br J Haematol 109(2): 280-87.
Vannucchi, A. M., F. Paoletti, et al. (2000). "Identification and characterization of a bipotent (erythroid and megakaryocytic) cell precursor from the spleen of phenylhydrazine-treated mice."
Blood 95(8): 2559-68.
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