Product Information

Contents: Functional Grade Biotin Rat IgG1 Isotype Control
Catalog Number: 36-4301
Sizes: 500 ug
Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, No NaN3. Endotoxin level is less than 0.05 ng/ug antibody, as determined by the LAL assay.
Storage Conditions: Store at 4°C. Do not freeze. Keep contents sterile.
Isotype: Rat IgG1, κ

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-4301	Affinity Purified Rat IgG1 Isotype Control	N/A	N/A	FC
16-4301	Functional Grade* Purified Rat IgG1 Isotype Control	N/A	N/A	FC
13-4301	Biotin Rat IgG1 Isotype Control	N/A	N/A	FC
11-4301	FITC Rat IgG1 Isotype Control	488	518	FC
12-4301	PE Rat IgG1 Isotype Control	488	575	FC
15-4301	Phycoerythrin-Cy5 (PE-Cy5) Rat IgG1 Isotype Control	488	670	FC
17-4301	APC Rat IgG1 Isotype Control	633	660	FC
19-4301	Cy5 Rat IgG1 Isotype Control	633	670	FC
25-4301	Phycoerythrin-Cy7 (PE-Cy7) Rat IgG1 Isotype Control	488	760	FC
36-4301	In Vivo Cytokine Capture Assay (IVCCA) In Vivo Capture Antibody – Control / Functional Grade* Biotin Rat IgG1 Isotype Control	N/A	N/A	IVCCA
51-4301	Alexa Fluor® 647 Rat IgG1 Isotype Control	633	668	FC
53-4301	Alexa Fluor® 488 Rat IgG1 Isotype Control	488	519	FC
56-4301	Alexa Fluor® 700 Rat IgG1 Isotype Control (APC-Cy5.5 Equivalent)	633	723	FC
57-4301	Pacific Blue® Rat IgG1 Isotype Control	402	421	FC
27-4301	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) Rat IgG1 Isotype Control (APC-Cy7 Equivalent)	633	779	FC
45-4301	PerCP-Cy5.5 Rat IgG1 Isotype Control	488	690	FC
47-4301	Allophycocyanin-eFluor^TM 780 Rat IgG1 Isotype Control (APC-Alexa Fluor® 750 replacement)	633	780	FC
48-4301	eFluor^TM 450 Rat IgG1 Isotype Control (Pacific Blue® replacement)	405	450	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The monoclonal rat IgG1, κ is useful as an isotype control immunoglobulin.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This Functional Grade Biotin Rat IgG1 Isotype Control has been reported for use as an isotype-matched control for in vivo capture assay.

Applications Tested

This Functional Grade Biotin Rat IgG1 Isotype Control has been sterility-tested and tested by LAL assay to verify low endotoxin. This Functional Grade Biotin Rat IgG1 Isotype Control is intended for use as an isotype control immunoglobulin for in vivo capture assay.

For detailed methodology in this application, please refer to this reference: Finkelman, F., S. Morris, T. Orekhova, and D. Sehy. 2003. The In Vivo Cytokine Capture Assay for measurement of cytokine production in the mouse. In Current Protocols in Immunology. Unit 6.28. J. Coligan, A. Kruisbeek, D. Margulies, E. Shevach, and W. Strober, eds. John Wiley and Sons, New York

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