Product Information

Contents: Functional Grade Biotin anti-mouse CD19
Catalog Number: 36-0191
Sizes: 500 ug
Formulation: Phosphate buffer pH 7.2,
500 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
Clone: MB19-1
Isotype: Mouse IgA, κ

Staining of C57Bl/6 splenocytes with staining buffer (autofluorescence) (open histogram) or 0.5 μg of Biotin anti-mouse CD19 (MB19-1) (colored histogram) followed by Streptavidin-PE (cat. 12-4312). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0191	FITC anti-mouse CD19	488	518	FC
12-0191	PE anti-mouse CD19	488	575	FC
15-0191	PE-Cy5 anti-mouse CD19	488	670	FC
17-0191	APC anti-mouse CD19	633	660	FC
36-0191	Functional Grade* Biotin anti-mouse CD19	N/A	N/A	FA FC IP
14-0191	Affinity Purified anti-mouse CD19	N/A	N/A	FA FC IP
16-0191	Functional Grade* Purified anti-mouse CD19	N/A	N/A	FA FC IP
13-0191	Biotin anti-mouse CD19	N/A	N/A	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The MB19-1 monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, MHC class II, and CD19 form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells. Staining of B cells with MB19-1 and its conjugates is usually dimmer than the rat anti-mouse CD19 antibody, clone 6D5.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This MB19-1 antibody has been reported for use in flow cytometric analysis, and immunoprecipitation.

Applications Tested

This MB19-1 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 2 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Engel, P., L. J. Zhou, et al. (1995). "Abnormal B lymphocyte development, activation, and differentiation in mice that lack or overexpress the CD19 signal transduction molecule." Immunity 3(1): 39-50.
Sato, S., N. Ono, et al. (1996). "CD19 regulates B lymphocyte signaling thresholds critical for the development of B-1 lineage cells and autoimmunity." J Immunol 157(10): 4371-8.
Sato, S., D. A. Steeber, et al. (1997). "CD19 expression levels regulate B lymphocyte development: human CD19 restores normal function in mice lacking endogenous CD19." J Immunol 158(10): 4662-9.
Tedder, T. F., M. Inaoki, et al. (1997). "The CD19-CD21 complex regulates signal transduction thresholds governing humoral immunity and autoimmunity." Immunity 6(2): 107-18.

Related Products

Cat. 12-0192    PE anti-mouse CD19 (clone 6D5)
Cat. 14-0192    Affinity Purified anti-mouse CD19 (clone 6D5)
Cat. 15-0192    PE-Cy5 anti-mouse CD19 (clone 6D5)
Cat. 16-0192    Functional Grade Purified anti-mouse CD19 (clone 6D5)
Cat. 30-0192    Allophycocyanin-Cy5.5 (APC-Cy5.5) anti-mouse CD19 (Alexa Fluor® 700 Equivalent) (clone 6D5)
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Cat. 36-4762    Functional Grade Biotin Mouse IgA Isotype Control