mouse CD4 (L3T4)

Contents: Functional Grade Biotin anti-mouse CD4 (L3T4)
Catalog Number: 36-0041
Formulation: Phosphate buffered saline, pH 7.2, No NaN₃
Storage Conditions: Store at 4°C.
Clone: GK1.5
Isotype: Rat IgG2b, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
36-0041-85	500 ug	$320

*International customers: Please contact your distributor for region specific pricing.

Staining of C57Bl/6 splenocytes with 0.125 μg of Biotin Rat IgG2b Iso Cntrl (cat. 13-4331) (open histogram) or 0.125 μg of Biotin anti-mouse CD4 (GK1.5) (colored histogram) followed by SAv-PE (cat. 12-4317). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0041	FITC anti-mouse CD4 (L3T4)	488	518	FC
12-0041	PE anti-mouse CD4 (L3T4)	488	575	FC
13-0041	Biotin anti-mouse CD4 (L3T4)	N/A	N/A	FC
14-0041	Affinity Purified anti-mouse CD4 (L3T4)	N/A	N/A	FC IH/F IP
15-0041	PE-Cy5 anti-mouse CD4 (L3T4)	488	670	FC
16-0041	Functional Grade* Purified anti-mouse CD4 (L3T4)	N/A	N/A	FA FC
17-0041	APC anti-mouse CD4 (L3T4)	633	660	FC
19-0041	Cy5 anti-mouse CD4 (L3T4)	633	670	FC
25-0041	PE-Cy7 anti-mouse CD4 (L3T4)	488	760	FC
36-0041	Functional Grade* Biotin anti-mouse CD4 (L3T4)	N/A	N/A	FC IH/F IP
51-0041	Alexa Fluor® 647 anti-mouse CD4 (L3T4)	633	668	FC
53-0041	Alexa Fluor® 488 anti-mouse CD4 (L3T4)	488	519	FC
56-0041	Alexa Fluor® 700 anti-mouse CD4 (L3T4) (APC-Cy5.5 Equivalent)	633	723	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of GK1.5 is blocked by RM4-5.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This GK1.5 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen tissue sections. It has also been reported in isolation and depletion of CD4+ cells, and for blocking of CD4-ligand binding and T cell activation that occurs through CD4.

Applications Tested

This GK1.5 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Mochimaru H, Usui T, Yaguchi T, Nagahama Y, Hasegawa G, Usui Y, Shimmura S, Tsubota K, Amano S, Kawakami Y, Ishida S. Suppression of alkali burn-induced corneal neovascularization by dendritic cell vaccination targeting VEGF receptor 2. Invest Ophthalmol Vis Sci. 2008 May;49(5):2172-7. (GK1.5, in vivo depletion, PubMed)

Yang Z, Day YJ, Toufektsian MC, Xu Y, Ramos SI, Marshall MA, French BA, Linden J. Myocardial infarct-sparing effect of adenosine A2A receptor activation is due to its action on CD4+ T lymphocytes. Circulation. 2006 Nov 7;114(19):2056-64. (GK1.5, in vivo depletion, PubMed)

Dialynas DP, Quan ZS, Wall KA, Pierres A, Quintáns J, Loken MR, Pierres M, Fitch FW. Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule. J Immunol. 1983 Nov;131(5):2445-51.

Wilde DB, Marrack P, Kappler J, Dialynas DP, Fitch FW. Evidence implicating L3T4 in class II MHC antigen reactivity; monoclonal antibody GK1.5 (anti-L3T4a) blocks class II MHC antigen- specific proliferation, release of lymphokines, and binding by cloned murine helper T lymphocyte lines. J Immunol. 1983 Nov;131(5):2178-83.

Related Products

Cat. 11-0042    FITC anti-mouse CD4 (L3T4) (clone RM4-5)
Cat. 12-0042    PE anti-mouse CD4 (L3T4) (clone RM4-5)
Cat. 13-0042    Biotin anti-mouse CD4 (L3T4) (clone RM4-5)
Cat. 14-0042    Affinity Purified anti-mouse CD4 (L3T4) (clone RM4-5)
Cat. 15-0042    PE-Cy5 anti-mouse CD4 (L3T4) (clone RM4-5)
Cat. 16-0042    Functional Grade Purified anti-mouse CD4 (L3T4) (clone RM4-5)
Cat. 17-0042    APC anti-mouse CD4 (L3T4) (clone RM4-5)
Cat. 25-0042    PE-Cy7 anti-mouse CD4 (L3T4) (clone RM4-5)
Cat. 30-0042    Allophycocyanin-Cy5.5 (APC-Cy5.5) anti-mouse CD4 (L3T4) (Alexa Fluor® 700 Equivalent) (clone RM4-5)
Cat. 35-0042    PE-Cy5.5 anti-mouse CD4 (L3T4) (clone RM4-5)
Cat. 11-0043    Fluorescein isothiocyanate (FITC) anti-mouse CD4 (L3T4) (clone RM4-4)
Cat. 12-0043    Phycoerythrin (PE) anti-mouse CD4 (L3T4) (clone RM4-4)
Cat. 13-0043    Biotin anti-mouse CD4 (L3T4) (clone RM4-4)
Cat. 14-0043    Affinity Purified anti-mouse CD4 (L3T4) (clone RM4-4)
Cat. 36-4031    In Vivo Cytokine Capture Assay (IVCCA) In Vivo Capture Antibody – Control / Functional Grade Biotin Rat IgG2b Isotype Control