Contents: Functional Grade Biotin anti-mouse CD3 (epsilon subunit; CD3e)
Catalog Number: 36-0031
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, No NaN3
Storage Conditions: Store at 4°C.
Clone: 145-2C11
Isotype: Armenian Hamster IgG
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Description
The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits, γ and δ, the ε chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 145-2C11 to TCR initiates the intracellular biochemical pathway resulting in cellular activation, proliferation, and apoptosis depending on specific conditions utilized. 145-2C11 is commonly used as a phenotypic marker for mouse T cells.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This 145-2C11 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistochemical staining of frozen tissue sections. It has also been reported for use in cell activation and cell depletion. Please visit the following website to view a protocol for
in vitro T-cell activation:
http://www.ebioscience.com/ebioscience/appls/AC145.htm
Applications Tested
This 145-2C11 antibody has been tested by flow cytometric analysis of mouse thymocyte and splenocyte suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Leo, O., M. Foo, et al. (1987). "Identification of a monoclonal antibody specific for a murine T3 polypeptide."
Proc Natl Acad Sci U S A 84(5): 1374-8.
Samelson, L. E., J. J. O'Shea, et al. (1987). "T cell antigen receptor phosphorylation induced by an anti-receptor antibody."
J Immunol 139(8): 2708-14.
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Mouse Cellular Antigens Chart