Product Information

Contents: Phycoerythrin-Cy5.5 (PE-Cy5.5) anti-mouse Ly-6A/E (Sca-1, Sca1)
Catalog Number: 35-5981
Sizes: 50 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: D7
Isotype: Rat IgG2a, κ

Surface staining of ConA stimulated splenocytes with anti-mouse Ly-6A/E (D7) PE (left), and APC (right). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5981	Affinity Purified anti-mouse Ly-6A/E (Sca-1, Sca1)	N/A	N/A	FC IP WB
16-5981	Functional Grade* Purified anti-mouse Ly-6A/E (Sca-1, Sca1)	N/A	N/A	FC
13-5981	Biotin anti-mouse Ly-6A/E (Sca-1, Sca1)	N/A	N/A	FC
11-5981	FITC anti-mouse Ly-6A/E (Sca-1, Sca1)	488	518	FC
12-5981	PE anti-mouse Ly-6A/E (Sca-1, Sca1)	488	575	FC
15-5981	PE-Cy5 anti-mouse Ly-6A/E (Sca-1, Sca1)	488	670	FC
17-5981	APC anti-mouse Ly-6A/E (Sca-1, Sca1)	633	660	FC
25-5981	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse Ly-6A/E (Sca-1, Sca1)	488	760	FC
35-5981	Phycoerythrin-Cy5.5 (PE-Cy5.5) anti-mouse Ly-6A/E (Sca-1, Sca1)	488	690	FC
56-5981	Alexa Fluor® 700 anti-mouse Ly-6A/E (Sca-1, Sca1) (APC-Cy5.5 Equivalent)	633	723	FC
45-5981	PerCP-Cy5.5 anti-mouse Ly-6A/E (Sca-1, Sca1) (PE-Cy5.5 replacement)	488	690	FC
93-5981	eFluor^TM 605^NC anti-mouse Ly-6A/E (Sca-1, Sca1)	350-500	605	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The D7 monoclonal antibody reacts with mouse Sca-1, an 18 kDa member of the Ly-6 family of GPI-linked surface proteins. D7 reacts with both Ly-6E.1 and Ly-6A.2 molecules expressed by mouse hematopoietic stem cells, myeloid population and peripheral T and B cells. Non-activated splenocytes from Ly-6.2 strains including C57BL, SJL, 129, AKR, and others demonstrate higher frequency of D7-positive cells compared to the Ly-6.1 strains such as BALB/c, C3H, NZB and others. Sca-1 is expressed at high levels upon activation regardless of the Ly-6 haplotype. The Ly-6 family is involved in regulation and function of T cell activation. Sca-1 is a major phenotypic marker for mouse hematopoietic progenitor/stem cell subset.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The D7 antibody has been reported for use in flow cytometric analysis.

NOTE: eBioscience will be discontinuing all PE-Cy5.5 conjugated antibodies. As an alternative, we recommend that customers use antibodies conjugated to PerCP-Cy5.5, which is a superior fluorochrome due to lesser requirement for compensation. Custom conjugations of PE-Cy5.5 will still be available upon request. Please contact eBioscience Technical Support for additional information.

Applications Tested

This D7 antibody has been tested by flow cytometric analysis of mouse splenocyte and bone marrow cell suspensions. This can be used at less than or equal to 0.06 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Ortega, G., P. E. Korty, et al. (1986). "Role of Ly-6 in lymphocyte activation. I. Characterization of a monoclonal antibody to a nonpolymorphic Ly-6 specificity." J Immunol 137(10): 3240-6.
Malek, T. R., G. Ortega, et al. (1986). "Role of Ly-6 in lymphocyte activation. II. Induction of T cell activation by monoclonal anti-Ly-6 antibodies." J Exp Med 164(3): 709-22.

Related Products

Cat. 35-4321  PE-Cy5.5 Rat IgG2a Isotype Control

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