Product Information

Contents: Phycoerythrin-Cy5.5 (PE-Cy5.5) anti-human CD45 (LCA)
Catalog Number: 35-0459
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: HI30
Host/Isotype: Mouse IgG1, κ
HLDA No.: IV N816

human CD45 antibody, Phycoerythrin-Cy5.5 (PE-Cy5.5) conjugate

Surface staining of normal human peripheral blood cells with anti-human CD45 (HI30) PE. Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0459	FITC anti-human CD45 (LCA)	488	518	FC
12-0459	PE anti-human CD45 (LCA)	488	575	FC
13-0459	Biotin anti-human CD45 (LCA)	N/A	N/A	FC
14-0459	Affinity Purified anti-human CD45 (LCA)	N/A	N/A	FC IHC
15-0459	PE-Cy5 anti-human CD45 (LCA)	488	670	FC
17-0459	Allophycocyanin (APC) anti-human CD45 (LCA)	633	660	FC
19-0459	Cy5 anti-human CD45 (LCA)	633	670	FC
25-0459	Phycoerythrin-Cy7 (PE-Cy7) anti-human CD45 (LCA)	488	760	FC
27-0459	DISCONTINUED - Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-human CD45 (LCA) (APC-Cy7 Equivalent)	633	779	FC
35-0459	Phycoerythrin-Cy5.5 (PE-Cy5.5) anti-human CD45 (LCA)	488	690	FC
45-0459	PerCP-Cy5.5 anti-human CD45 (LCA) (PE-Cy5.5 replacement)	488	690	FC
47-0459	Allophycocyanin-eFluor® 780 anti-human CD45 (LCA)	633	780	FC
48-0459	eFluor® 450 anti-human CD45 (LCA)	405	450	FC
57-0459	DISCONTINUED - Refer to NEW eFluor® 450 anti-human CD45 (Pacific Blue® replacement) (LCA)	402	421	FC
93-0459	eFluor® 605NC anti-human CD45 (LCA)	350-500	605	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The HI30 monoclonal antibody reacts with all isoforms of human CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The HI30 antibody has been reported for use in flow cytometric analysis.

NOTE: eBioscience will be discontinuing all PE-Cy5.5 conjugated antibodies. As an alternative, we recommend that customers use antibodies conjugated to PerCP-Cy5.5, which is a superior fluorochrome due to lesser requirement for compensation. Custom conjugations of PE-Cy5.5 will still be available upon request. Please contact eBioscience Technical Support for additional information.

Applications Tested

The HI30 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.

Related Products

Cat. 35-4714 Phycoerythrin-Cy5.5 (PE-Cy5.5) Mouse IgG1, K Isotype Control

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