PE-Cy5.5 anti-human CD22 antibody: Phycoerythrin-Cy5.5 (Cy5.5PE) conjugate

Print Page

Adobe PDF

Contents: Phycoerythrin-Cy5.5 (PE-Cy5.5) anti-human CD22
Catalog Number: 35-0229
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBio4KB128 (4KB128)
Isotype: Mouse IgG1, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
35-0229-71	25 tests	$145
35-0229-73	100 tests	$295

*International customers: Please contact your distributor for region specific pricing.

Staining of normal human peripheral blood cells with FITC anti-human CD19 (HIB19) (cat. 11-0199) and 0.06 μg of PE-Cy5.5 Mouse IgG1, K Iso Cntrl (cat. 35-4714) (left) or PE-Cy5.5 anti-human CD22 (4KB128) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-0229	Fluorescein isothiocyanate (FITC) anti-human CD22	488	518	FC
12-0229	Phycoerythrin (PE) anti-human CD22	488	575	FC
15-0229	Phycoerythrin-Cy5 (PE-Cy5) anti-human CD22	488	670	FC
35-0229	Phycoerythrin-Cy5.5 (PE-Cy5.5) anti-human CD22	488	690	FC
14-0229	Affinity Purified anti-human CD22	N/A	N/A	FC IHC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eBio4KB128 monoclonal antibody recognizes human CD22 (Siglec-2), which is a member of the siglec subgroup of the Ig superfamily. CD22 is a type I transmembrane glycoprotein composed of two polypeptide chains, CD22α and CD22β, of 130 and 140 kDa respectively, produced by alternative splicing of the CD22 gene. CD22 is expressed at high levels on mature B cells and B cell lymphomas. The extracellular portion of CD22 contains seven Ig-like domains, some of which are capable of binding ligands with sialic acid moieties expressed on epithelial, endothelial, B and T cells. The intracellular portion of CD22 contains 6 tyrosine residues contained within immunotyrosine-based inhibitory motifs (ITIM) and immunotyrosine-based activation-like motifs, which are phosphorylated upon B-cell receptor engagement, which enables CD22 to participate in the positive and negative regulation of B-cell receptor signaling.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBio4KB128 (4KB128) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBio4KB128 (4KB128) antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood cells. This can be used at 20 μl (0.06 μg) per million cells in a 100 μl total staining volume.

References

Mason DY, Stein H, Gerdes J, Pulford KA, Ralfkiaer E, Falini B, Erber WN, Micklem K, Gatter KC. Value of monoclonal anti-CD22 (p135) antibodies for the detection of normal and neoplastic B lymphoid cells. Blood. 1987 Mar;69(3):836-40. (4KB128, IHC, PubMed)

Campana D, Janossy G, Bofill M, Trejdosiewicz LK, Ma D, Hoffbrand AV, Mason DY, Lebacq AM, Forster HK. Human B cell development. I. Phenotypic differences of B lymphocytes in the bone marrow and peripheral lymphoid tissue. J Immunol. 1985 Mar;134(3):1524-30.

Nitschke L. The role of CD22 and other inhibitory co-receptors in B-cell activation. Curr Opin Immunol. 2005 Jun;17(3):290-7. Review.

Related Products

Cat. 35-4714    Phycoerythrin-Cy5.5 (PE-Cy5.5) Mouse IgG1, K Isotype Control

FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APLICATIONS. OTHER USE NEEDS LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC. UNDER U.S. PATENT Nos. 4,981,977 AND 5,268,486 AND OTHER PATENTS PENDING.

THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF AMERSHAM PHARMACIA BIOTECH INC. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC.  THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH.   IT IS NOT LICENSED FOR ANY OTHER USE.    THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.   COMMERCIAL USE shall include:

1.         Sale, lease, license or other transfer of the material or any material derived or produced from it.
2.         Sale, lease, license or other grant of rights to use this material or any other material derived or produced from it
3.         Use of this material to perform services for a fee for third parties.

IF YOU REQUIRE A COMMERCIAL LICENSE TO USE THIS MATERIAL AND DO NOT HAVE ONE, RETURN THIS MATERIAL, UNOPENED TO eBioscience AND ANY MONEY PAID FOR THE MATERIAL WILL BE REFUNDED.