Product Information

Contents: Phycoerythrin-Cy5.5 (PE-Cy5.5) anti-mouse CD3 (epsilon subunit; CD3e)
Catalog Number: 35-0031
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 145-2C11
Isotype: Armenian Hamster IgG

Surface staining of mouse splenocytes with anti-mouse CD3e (145-2C11) FITC (left), and PE (right). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0031	Affinity Purified Hamster anti-mouse CD3 (epsilon subunit; CD3e)	N/A	N/A	FA FC IH/F IP WB
16-0031	Functional Grade* Purified Hamster anti-mouse CD3 (epsilon subunit; CD3e)	N/A	N/A	FA FC
13-0031	Biotin Hamster anti-mouse CD3 (epsilon subunit; CD3e)	N/A	N/A	FC
11-0031	FITC Hamster anti-mouse CD3 (epsilon subunit; CD3e)	488	518	FC
12-0031	PE Hamster anti-mouse CD3 (epsilon subunit; CD3e)	488	575	FC
15-0031	PE-Cy5 Hamster anti-mouse CD3 (epsilon subunit; CD3e)	488	670	FC
17-0031	APC Hamster anti-mouse CD3 (epsilon subunit; CD3e)	633	660	FC
25-0031	PE-Cy7 Hamster anti-mouse CD3 (epsilon subunit; CD3e)	488	760	FC
36-0031	Functional Grade* Biotin anti-mouse CD3 (epsilon subunit; CD3e)	N/A	N/A	FA FC IH/F IP WB
53-0031	Alexa Fluor® 488 anti-mouse CD3 (epsilon subunit; CD3e)	488	519	FC
45-0031	PerCP-Cy5.5 anti-mouse CD3 (epsilon subunit; CD3e) (PE-Cy5.5 replacement)	488	690	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Custom Product Requests
Need a custom product? Download the Custom Product Request Form and submit completed form to customs@ebioscience.com.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits, γ and δ, the ε chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 145-2C11 to TCR initiates the intracellular biochemical pathway resulting in cellular activation, proliferation, and apoptosis depending on specific conditions utilized. 145-2C11 is commonly used as a phenotypic marker for mouse T cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 145-2C11 antibody has been reported for use in flow cytometric analysis.

NOTE: eBioscience will be discontinuing all PE-Cy5.5 conjugated antibodies. As an alternative, we recommend that customers use antibodies conjugated to PerCP-Cy5.5, which is a superior fluorochrome due to lesser requirement for compensation. Custom conjugations of PE-Cy5.5 will still be available upon request. Please contact eBioscience Technical Support for additional information.

Applications Tested

The 145-2C11 antibody has been tested by flow cytometric analysis of mouse thymocyte and splenocyte suspensions. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Geng H, Zhang GM, Xiao H, Yuan Y, Li D, Zhang H, Qiu H, He YF, Feng ZH. HSP70 vaccine in combination with gene therapy with plasmid DNA encoding sPD-1 overcomes immune resistance and suppresses the progression of pulmonary metastatic melanoma. Int J Cancer. 2006 Jun 1;118(11):2657-64. (145-2C11, IHC frozen, PubMed)

Krieg C, Han P, Stone R, Goularte OD, Kaye J. Functional analysis of B and T lymphocyte attenuator engagement on CD4+ and CD8+ T cells. J Immunol. 2005 Nov 15;175(10):6420-7. (145-2C11, FA, PubMed)

Takeuchi A, Usui Y, Takeuchi M, Hattori T, Kezuka T, Suzuki J, Okunuki Y, Iwasaki T, Haino M, Matsushima K, Usui M. CCR5-deficient mice develop experimental autoimmune uveoretinitis in the context of a deviant effector response. Invest Ophthalmol Vis Sci. 2005 Oct;46(10):3753-60. (145-2C11, IHC frozen, PubMed)

Schuchert MJ, Wright RD, Colson YL. Characterization of a newly discovered T-cell receptor beta-chain heterodimer expressed on a CD8+ bone marrow subpopulation that promotes allogeneic stem cell engraftment. Nat Med. 2000 Aug;6(8):904-9. (145-2C11, WB and IP)

Kearse KP. Calnexin associates with monomeric and oligomeric (disulfide-linked) CD3delta proteins in murine T lymphocytes. J Biol Chem. 1998 Jun 5;273(23):14152-7. (145-2C11, IP and WB)

Samelson LE, O'Shea JJ, Luong H, Ross P, Urdahl KB, Klausner RD, Bluestone J. T cell antigen receptor phosphorylation induced by an anti-receptor antibody. J Immunol. 1987 Oct 15;139(8):2708-14.

Leo O, Foo M, Sachs DH, Samelson LE, Bluestone JA. Identification of a monoclonal antibody specific for a murine T3 polypeptide.Proc Natl Acad Sci U S A. 1987 Mar;84(5):1374-8.

Related Products

Cat. 35-4888  Phycoerythrin-Cy5.5 (PE-Cy5.5) Armenian Hamster IgG Isotype Control (clone eBio299Arm)

FOR NON-COMMERCIAL RESEARCH USE ONLY. NOT FOR THERAPEUTIC OR IN VIVO APLICATIONS. OTHER USE NEEDS LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC. UNDER U.S. PATENT Nos. 4,981,977 AND 5,268,486 AND OTHER PATENTS PENDING.

THIS MATERIAL IS SUBJECT TO PROPRIETARY RIGHTS OF AMERSHAM PHARMACIA BIOTECH INC. AND CARNEGIE MELLON UNIVERSITY AND MADE AND SOLD UNDER LICENSE FROM AMERSHAM PHARMACIA BIOTECH INC.  THIS PRODUCT IS LICENSED FOR SALE ONLY FOR RESEARCH.   IT IS NOT LICENSED FOR ANY OTHER USE.    THERE IS NO IMPLIED LICENSE HEREUNDER FOR ANY COMMERCIAL USE.   COMMERCIAL USE shall include:

1.         Sale, lease, license or other transfer of the material or any material derived or produced from it.
2.         Sale, lease, license or other grant of rights to use this material or any other material derived or produced from it
3.         Use of this material to perform services for a fee for third parties.

IF YOU REQUIRE A COMMERCIAL LICENSE TO USE THIS MATERIAL AND DO NOT HAVE ONE, RETURN THIS MATERIAL, UNOPENED TO eBioscience AND ANY MONEY PAID FOR THE MATERIAL WILL BE REFUNDED.