Carrier-Free Recombinant Mouse Chemerin

Contents: Carrier-Free Recombinant Mouse Chemerin
Catalog Number: 34-8951
Formulation: Sterile liquid; phosphate buffered saline, pH 7.2, 150 mM NaCl, with no carrier or stabilizer, 0.22 µM filtered.
Storage Conditions: For greatest stability, keep concentration of primary stock at or above 10 µg/ml. For long term storage, aliquot into polypropylene vials (volumes of 20 µl or greater) and store at or below -80°C. Avoid repeated freeze/thaw cycles.
Handling Conditions: For best recovery, always quick-spin vial prior to opening. For dilution of current stock, always include carrier protein (1% BSA or 10% FBS) in the buffered saline diluent.
Source: E. coli expressed amino acids Thr17 - Ser156 of mouse chemerin (accession # NP_082128).
Molecular Mass: Mature mouse chemerin protein has a predicted molecular mass of approximately 16,000 kDa.
Purity: Greater than 98%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.01 ng/ug cytokine, as determined by the LAL assay.
Bioactivity: Measured in a bioassay of chemerin-induced migration of CHO cells transfected with the mouse chemerin receptor, CMKLR. Maximal chemotactic activity of chemerin in this assay is typically observed at 50-100 ng/ml.

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
34-8951-82	100 ug	$1800

*International customers: Please contact your distributor for region specific pricing.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
34-8951	Carrier-Free Recombinant Mouse Chemerin	N/A	N/A	BA
14-8951	Recombinant Mouse Chemerin	N/A	N/A	BA

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

Chemerin is a potent chemoattractant, identified as the ligand of CMKLR (also known as Chem23R, DEZ), a receptor expressed by plasmacytoid DCs, and some macrophage and NK cell subsets. Pro-chemerin circulates in the blood and is biologically inactive, until processed by a variety of serine proteases at sites of tissue damage to remove six C-terminal amino acid residues. The cleaved, mature form of chemerin is biologically active and recruits CMKLR1-positive cells to sites of tissue damage. Chemerin is a member of the cystatin superfamily and contains three intrachain disulfide bonds.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The recombinant mouse chemerin has been reported useful for bioassay.

Applications Tested

This recombinant mouse chemerin has been tested in bioassay of migration of CHO cells transfected with the mouse chemerin receptor, CMKLR (Zabel, et al, 2005. J. Biol. Chem. 280: 34661). Maximal chemotactic activity of chemerin in this assay is typically observed at 50-100 ng/ml.

References

Wittamer V, Franssen JD, Vulcano M, Mirjolet JF, Le Poul E, Migeotte I, Brezillon S, Tyldesley R, Blanpain C, Detheux M, Mantovani A, Sozzani S, Vassart G, Parmentier M, Communi D. Specific recruitment of antigen-presenting cells by chemerin, a novel processed ligand from human inflammatory fluids. J Exp Med. 2003 Oct 6;198(7):977-85. (PubMed)

Zabel BA, Silverio AM, Butcher EC. Chemokine-like receptor 1 expression and chemerin-directed chemotaxis distinguish plasmacytoid from myeloid dendritic cells in human blood. J Immunol. 2005 Jan 1;174(1):244-51. (PubMed)

Zabel BA, Allen SJ, Kulig P, Allen JA, Cichy J, Handel TM, Butcher EC. Chemerin activation by serine proteases of the coagulation, fibrinolytic, and inflammatory cascades. J Biol Chem. 2005 Oct 14;280(41):34661-6. (PubMed)

Zabel BA, Ohyama T, Zuniga L, Kim JY, Johnston B, Allen SJ, Guido DG, Handel TM, Butcher EC. Chemokine-like receptor 1 expression by macrophages in vivo: regulation by TGF-beta and TLR ligands. Exp Hematol. 2006 Aug;34(8):1106-14. (PubMed)

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