Human TSLP Recombinant Protein Carrier-Free

Also known as: Thymic Stromal-Derived Lymphopoietin

RUO: For Research Use Only

Contents: Human TSLP Recombinant Protein Carrier-Free
Catalog Number: 34-8497
Formulation: Sterile liquid; 20 mM NaH2PO4, 0.6M NaCl, pH 7.2. 0.22 µm filtered.
Storage Conditions: Store at less than or equal to -70°C.
Handling Conditions: For best recovery, quick-spin vial prior to opening. Use in sterile envrioment.
Source: Insect cell expressed amino acids Y29-Q159 of hTSLP (accession #NM_033035).
Molecular Mass: 15 kDa on SDS-PAGE
Purity: Greater than 95%, as determined by SDS-PAGE
Endotoxin Level: Less than 0.01 ng/ug cytokine as determined by the LAL assay.
Bioactivity: Measured by TARC induction assay by human PBMCs with an observed ED₅₀ of 10 ng/ml, which corresponds to a specific activity of approximately 1x10⁵ Units/mg.

Product Options
Cat. No.	Size	Price	Add Qty to Cart
34-8497-82	100 ug	To view relevant pricing, please select your country and resume browsing.
34-8497-85	500 ug

Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.

Research Related Products
Cat. No.	Format
14-8497	Human TSLP Recombinant Protein
88-7497	Human TSLP ELISA Ready-SET-Go!® Set

Available Formats
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications	Regulatory Status
14-8497	Human TSLP Recombinant Protein	N/A	N/A	BA	RUO
34-8497	Human TSLP Recombinant Protein Carrier-Free	N/A	N/A	BA	RUO
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.

Description

Thymic Stromal Lymphopoietin (TSLP) is a member of the B cell-stimulating factor family. This hemopoietic cytokine was identified from the conditioned medium of a mouse thymic stromal cell line that promoted B-cell development. TSLP is expressed in thymus, spleen, kidney, lung and bone marrow. The bioactivity of TSLP overlaps with that of IL-7, playing a key role in B cell development, as well as stimulating the growth of thymocytes and T cells. Whereas IL-7 facilitates the development of B220+/IgM- pre-B cells, TSLP promotes the development B220+/IgM+ B cells in the mouse. In addition, TSLP stimulates monocytes to produce the T cell-attracting chemokines TARC (CCL17) and MDC (CCL22). TSLP also activates dendritic cell-primed CD4 T cells to produce Th2 cytokines (e.g. IL-4, IL-5, IL-13, and TNFα). TSLP binds to a heterodimeric receptor complex consisting of the IL-7R alpha chain (IL-7Rα) and the TSLP-specific chain (TSLPR) to induce STAT3 and STAT5 phosphorylation. Mouse TSLP shares approximately 43% amino acid sequence identity with human TSLP. Recombinant human TSLP is a 15.0 kDa protein consisting of 121 amino acid residues.

Applications Reported

For research use only, not for diagnostic or therapeutic use. Recombinant human TSLP is biologically active.

Applications Tested

This recombinant human TSLP has been tested for TARC induction by normal human peripheral blood cells with an observed ED₅₀ of 10 ng/ml, which corresponds to a specific activity of approximately 1x10⁵ Units/mg.

References

He R, Oyoshi MK, Garibyan L, Kumar L, Ziegler SF, Geha RS. TSLP acts on infiltrating effector T cells to drive allergic skin inflammation. Proc Natl Acad Sci U S A. 2008 Aug 19;105(33):11875-80.

Soumelis V, Reche PA, Kanzler H, Yuan W, Edward G, Homey B, Gilliet M, Ho S, Antonenko S, Lauerma A, Smith K, Gorman D, Zurawski S, Abrams J, Menon S, McClanahan T, de Waal-Malefyt Rd R, Bazan F, Kastelein RA, Liu YJ. Human epithelial cells trigger dendritic cell mediated allergic inflammation by producing TSLP. Nat Immunol. 2002 Jul;3(7):673-80.

Sims JE, Williams DE, Morrissey PJ, Garka K, Foxworthe D, Price V, Friend SL, Farr A, Bedell MA, Jenkins NA, Copeland NG, Grabstein K, Paxton RJ. Molecular cloning and biological characterization of a novel murine lymphoid growth factor. J Exp Med. 2000 Sep 4;192(5):671-80.

Park LS, Martin U, Garka K, Gliniak B, Di Santo JP, Muller W, Largaespada DA, Copeland NG, Jenkins NA, Farr AG, Ziegler SF, Morrissey PJ, Paxton R, Sims JE. Cloning of the murine thymic stromal lymphopoietin (TSLP) receptor: Formation of a functional heteromeric complex requires interleukin 7 receptor. J Exp Med. 2000 Sep 4;192(5):659-70.

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Unless indicated otherwise, all products are For Research Use Only. Not for use in diagnostic or therapeutic procedures. Not for further distribution without written consent. Not all products available in all regions.