Contents: Carrier-Free Recombinant Mouse HGF (Hepatocyte growth factor)
Catalog Number: 34-8431
Formulation: Sterile liquid; phosphate buffered saline, pH 7.2, 150 mM NaCl, with no carriers or preservatives. 0.22 µm filtered.
Storage Conditions: For greatest stability, keep concentration of primary stock at or above 10 µg/ml. For long term storage, aliquot into polypropylene vials (volumes of 20 µl or greater) and store at or below -80°C. Avoid repeated freeze/thaw cycles.
Handling Conditions: For best recovery, always quick-spin vial prior to opening. For dilution of current stock, always include carrier protein (1% BSA or 10% FBS) in the buffered saline diluent.
Source: Insect cells infected with baculovirus: amino acids 1-728 of mouse HGF (accession # D10212).
Molecular Mass: The intact mature protein, amino acids gln33-leu728, has a predicted molecular mass of 79,375. The protein migrates as an 85 kDa polypeptide on reducing SDS-PAGE due to glycosylation and as a 66 kDa polypeptide on non-reducing SDS-PAGE due to internal cystines.
Purity: Greater than 98% as determined by SDS-PAGE
Endotoxin Level: Less than 0.01 ng/ug cytokine as determined by the LAL assay.
Bioactivity: Measured in a bioassay of dose-dependent stimulation of mIMCD-3 cell proliferation. The ED50 for this is typically below 20 ng/ml, corresponding to a specific activity of greater than 5.0 x 104 U/mg.
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Description
Hepatocyte Growth Factor (HGF), also known as Hepatopoietin A and Scatter Factor, was originally identified based on its mitogenic activity for hepatocytes. Plasma from patients with hepatic failure was found to contain a factor that stimulates the growth of rat hepatocytes in primary culture. This plasma-derived HGF was purified and found to have multiple forms with molecular weights between 76 kD and 92 kD. Mature HGF consists of 2 disulfide-linked chains, heavy and light, with molecular weights of 54-65 kD and 31.5-34.5 kD, respectively. The heavy and light chains are encoded by the same mRNA, which by translation yields a biologically inactive single chain pro-peptide. The pro-peptide is cleaved by an extracellular serum serine protease to form the bioactive disulfide-linked alpha and beta chain heterodimeric HGF. HGF has also been described as Scatter Factor, based on its capacity to stimulate the dissociation and scattering of epithelial cells. The sequence of HGF has about 35% identity to that of plasminogen and the putative cleavage site of HGF is identical to that of plasminogen. The HGF receptor gene, the MET oncogene, is located on 7q, along with the HGF gene. An increase in the copy number of chromosome 7 is one of the most common chromosome abnormalities observed in human malignant gliomas.
Applications Reported
For research use only, not for diagnostic or therapeutic use.
Applications Tested
This recombinant mouse HGF has been tested in a bioassay of dose-dependent stimulation of mIMCD-3 cell proliferation. The ED
50 for this is typically 20 ng/ml, corresponding to a specific activity of 5.0 x 10
4 U/mg.
References
Gohda, E., et al. 1988. J. Clin. Invest. 81: 414-419.
Weidner, KM., et al. 1991. Proc. Nat. Acad. Sci. 88: 7001-7005.
Zarnegar, R., et al. 1992. Genomics 12: 147-150.
Birchmeier, C., et al. 2003. Nature Reviews Mol Cell Biol. 4: 915.
