Contents: Carrier-Free Recombinant Human GM-CSF
Catalog Number: 34-8339
Formulation: Sterile liquid; 10 mM sodium phosphate, pH 7.2, 150 mM NaCl, with no carrier or stabilizer. 0.22 uM filtered.
Storage Conditions: For greatest stability, keep concentration of primary stock at or above 10 µg/ml. For long term storage, aliquot into polypropylene vials (volumes of 20 µl or greater) and store at or below -80°C. Avoid repeated freeze/thaw cycles.
Handling Conditions: For best recovery, always quick-spin vial prior to opening. For dilution of current stock, always include carrier protein (1% BSA or 10% FBS) in the buffered saline diluent.
Source: E. coli expressed amino acids ala 18 - glu144 of mature human GMCSF (accession # NM_000758).
Molecular Mass: The protein is not methionylated at the N-terminal and has a predicted molecular mass of 14,478. The DTT reduced protein migrates as a 14 kDa polypeptide on SDS-PAGE. The non-reduced protein migrates as a 13 kDa polypeptide.
Purity: Greater than 98%, as determined by SDS-PAGE
Endotoxin Level: Less than 0.01 ng/ug cytokine as determined by the LAL assay.
Bioactivity: Measured by human TF-1 cell proliferation assay. The ED50 is 0.1 ng/ml, corresponding to a specific activity of 1.0 x 107 Units/mg.
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Description
Human GM-CSF (Granulocyte/Monocyte-Colony Stimulating Factor) is a differentially glycosylated factor produced mainly by activated T cells and macrophages. Endothelial cells and fibroblasts can also produce GM-CSF after exposure to TNF-α, IL-1, IL-2 and IFN-γ. GM-CSF is found associated with extracellular matrix and in membrane-bound formats too. GM-CSF stimulates proliferation, activation and differentiation of macrophages and granulocytes and their progenitors.
Applications Reported
For research use only, not for diagnostic or therapeutic use. Recombinant human GM-CSF is biologically active and can promote proliferation of the human TF-1 cell line in culture. It is also useful as a standard for a human GM-CSF ELISA.
Applications Tested
This recombinant human GM-CSF has been tested in bioassays using human TF-1 cell line. The ED
50 measured in a TF-1 proliferation assay is typically 100 pg/ml, corresponding to a specific activity of approximately 1.0 x10
7 Units/mg.
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