Contents: Carrier-Free Recombinant human IL-33, Interleukin 33
Catalog Number: 34-8338
Formulation: Sterile liquid; 10 mM sodium phosphate, pH 7.2, 150 mM NaCl. 0.22 µm filtered.
Storage Conditions: For greatest stability, keep concentration of primary stock at or above 10 µg/ml. For long term storage, aliquot into polypropylene vials (volumes of 20 µl or greater) and store at or below -80°C. Avoid repeated freeze/thaw cycles.
Handling Conditions: For best recovery, always quick-spin vial prior to opening. For dilution of current stock, always include carrier protein (1% BSA or 10% FBS) in the buffered saline diluent.
Source: E. coli expressed protein consisting of amino acids Ser 112-Thr 270 of mature human IL-33 (accession # NM-033439).
Molecular Mass: The DTT reduced protein migrates as a 18 kDa polypeptide on SDS-PAGE.
Purity: Greater than 98%, as determined by SDS-PAGE
Endotoxin Level: Less than 0.01 ng/ug cytokine as determined by the LAL assay.
Bioactivity: Measured by D10.G4.1 cell proliferation assay. The ED50 is 0.3 ng/ml, corresponding to a specific activity of 3.0 x 106 Units/mg.
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Description
Human IL-33, also called NF-HEV and DVS 27, is a 30 kDa proinflammatory cytokine produced by smooth muscle, epithelia and fibroblasts upon stimulation. IL-33 can be cleaved by caspase-1 and the C-terminal fragment is the mature IL-33. IL-33 was recently identified as the ligand for T1/ST2 (ST2). ST2 is stably expressed on mast cells and T(h)2 effector T cells and is functionally associated with T(h)2-inflammation. IL-33 plays an immune regulatory role by inducing IL-5 and IL-13
in vitro and
in vivo. Human IL-33 shares 55% amino acid sequence identity with mouse.
Applications Reported
For research use only, not for diagnostic or therapeutic use. Carrier-Free Recombinant human IL-33 has been reported for use in cytokine bioassays.
Applications Tested
This recombinant human IL-33 has been tested by bioassays using the cell line D10.G4.1. The ED50 measured in the D10.G4.1 proliferation assay is typically less than 1 ng/ml, corresponding to a specific activity of greater than 1x106 Units/mg.
References
Schmitz, J., et al. 2005. IL-33, an interleukin-1-like cytokine that signals via the IL-1 receptor-related protein ST2 and induces T helper type 2-associated cytokines. Immunity 23: 479-490.
Verri, W. A. Jr., et al. 2008. IL-33 mediates antigen-induced cutaneous and articular hypernociception in mice. Proc. Nat. Acad. Sci. 105: 2723-2728.
