Welcome to eBioscience. Tel: 888.999.1371 or 858.642.2058.  San Diego, California, USA.
Home BestProtocols® Technical Support About Us Contact Us
Clinical Products Research Products Custom Products Weekly New Products

Choose language for TDS:
 

Human IFN alpha 1 Recombinant Protein Carrier-Free

Also known as: Interferon-alpha1, IFN-a1

RUO: For Research Use Only
 
Contents: Human IFN alpha 1 Recombinant Protein Carrier-Free
Catalog Number: 34-8318
Formulation: Sterile liquid; phosphate buffered saline, pH 7.2, 150 mM NaCl, with no carriers or preservatives. 0.22 µm filtered.
Storage Conditions: Store at less than or equal to -70°C.
Handling Conditions: For best recovery, quick-spin vial prior to opening. Use in sterile envrioment.
Source: E. coli expressed amino acids Cys 24-Asp 189 of mature human IFNα1 (accession # NM_024013).
Molecular Mass: The protein is not methionylated at the N-terminal and has a predicted molecular mass of 19,386. The DTT reduced protein migrates as a 21 kDa polypeptide on SDS-PAGE and the non-reduced protein migrates as a 16 kDa polypeptide due to intramolecular disulfides.
Purity: Greater than 98% as determined by SDS-PAGE
Endotoxin Level: Less than 0.01 ng/ug cytokine as determined by the LAL assay.
Bioactivity: The recombinant human IFN-α1 has been tested for inhibition of the cytopathic effect of EMC virus on A549 cells. The ED50 for this effect is typically below 0.3 ng/ml, corresponding to a specific activity of 3 x 106 U/mg.
 
Product Options
Cat. No. Size Price Add Qty to Cart
34-8318-82 100 ug
To view relevant pricing, please select your country and resume browsing.

34-8318-85 500 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
   
 

Available Formats
Cat. No.FormatExcite
(nm)
Emit
(nm)
Reported ApplicationsRegulatory Status
14-8318 Human IFN alpha 1 Recombinant Protein N/A N/A BA RUO
34-8318 Human IFN alpha 1 Recombinant Protein Carrier-Free N/A N/A BA RUO
39-8318 Human IFN alpha 1 Single-Use ELISA RSG Standard N/A N/A ELISA std RUO
Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
 
Description
Human IFN-α1 is a type I interferon previously known as B-cell interferon, leukocyte interferon, lymphoblast interferon, and pH2-stable interferon. IFN-α1 is one of at least 23 different known variants of IFN-α. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. The IFN-α forms are produced by monocytes/macrophages, lymphoblastoid cells, fibroblasts, and other cell types following induction by viruses, nucleic acids, glucocorticoid hormones, and low-molecular weight substances (n-butyrate, 5-bromodeoxyuridine). Human IFN-α1 promotes antiviral, antiparasitic, and antiproliferative activities.
Applications Reported
For research use only, not for diagnostic or therapeutic use. Recombinant human IFN-α1 is biologically active.
Applications Tested
The recombinant human IFN-α1 has been tested for inhibition of the cytopathic effect of EMC virus on A549 cells. The ED50 for this effect is typically below 0.3 ng/ml, corresponding to a specific activity of 3 x 106 U/mg.
References
Nagai, T., et al. 2003. Timing of IFN-beta exposure during human dendritic cell maturation and naive Th cell stimulation has contrasting effects on Th1 subset generation: a role for IFN-beta-mediated regulation of IL-12 family cytokines and IL-18 in naive Th cell differentiation. J. Immunol. 171: 5233-43.

Home  |   Index  |   Site Map  |   FAQ  |   Terms & Conditions  |   Distributors  |   Careers  |   Contact Us
Copyright © 2000-2010 eBioscience, Inc.
Unless indicated otherwise, all products are For Research Use Only. Not for use in diagnostic or therapeutic procedures. Not for further distribution without written consent. Not all products available in all regions.