Contents: Carrier-Free Recombinant Mouse IFNg (Interferon-gamma, IFN-g) Catalog Number: 34-8311 Formulation: Sterile 10 mM NaH2PO4, 150 mM NaCl, 1 mM DTT, with no carriers or preservatives. 0.22 µm filtered. Storage Conditions: For greatest stability, keep concentration of primary stock at or above 10 µg/ml. For long term storage, aliquot into polypropylene vials (volumes of 20 µl or greater) and store at or below -80°C. Avoid repeated freeze/thaw cycles. Handling Conditions: For best recovery, always quick-spin vial prior to opening. For dilution of current stock, always include carrier protein (1% BSA or 10% FBS) in the buffered saline diluent. Source: E. coli expressed amino acids His 23-Cys 155 of mouse IFN-γ (accession # NM_008337). Molecular Mass: The protein is methionylated at the N-terminal. The DTT reduced and protein migrates as a 14.5 kDa polypeptides on SDS-PAGE. Purity: Greater than 98%, as determined by SDS-PAGE Endotoxin Level: Less than 0.01 ng/ug cytokine as determined by the LAL assay. Bioactivity: Measured by mouse L929/EMCV cell protection assay. The ED50 is 100 pg/ml, corresponding to a specific activity of 1.0 x 10E7 Units/mg.
Product Options
Cat. No.
Size
Price
Add Qty to Cart
34-8311-82
100 ug
34-8311-85
500 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.
Description
Mouse IFN-γ is a ~20 kDa factor produced by activated T, B and NK cells, and is an anti-viral and anti-parasitic cytokine. IFN-γ, in synergy with other cytokines such as TNF-α, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN-γ are exerted on a wide range of cell types expressing the high affinity receptors for IFN-γ. Glycosylation of IFN-γ does not affect its biological activity.
Applications Reported
For research use only, not for diagnostic or therapeutic use. Recombinant mouse IFN-γ is biologically active and is for bioassay use only. For an ELISA standard, please refer to eBioscience Cat. No. 39-8311-65.
Applications Tested
This reagent has been tested in bioassays using the mouse fibroblast cell line L929. The ED50 measured in a L929 protection is typically 100 pg/ml, corresponding to a specific activity of approximately 1x107 Units/mg.
