Product Information

Contents: Carrier-Free Recombinant Human IL-21 (Interleukin-21, IL21)
Catalog Number: 34-8219
Sizes: 100 ug, 500 ug
Concentration: 0.5 mg/mL
Formulation: Sterile liquid; 20 mM sodium phosphate, pH 7.2, 0.5 M NaCl. 0.22 um filtered.
Storage Conditions: For greatest stability, keep concentration of primary stock at or above 10 ug/ml. For long term storage, aliquot and store at or below -80oC. Avoid repeated freeze/thaw cycles.
Handling Conditions: For best recovery, always quick-spin vial prior to opening. For dilution of current stock, always include carrier protein (1% BSA or 10% FBS) in the buffered saline diluent.
Source: A DNA sequence encoding the mature form of human IL-21amino acids Gln25-Ser155 (accession # NM_021803) was expressed in E.coli
Molecular Mass: The protein has a predicted molecular mass of 17,150. The DTT reduced protein migrates as a 18 kDa polypeptide on SDS-PAGE. The non-reduced protein migrates as a 17 kDa polypeptide.
Purity: Greater than 98% as determined by SDS-PAGE.
Endotoxin Level: Less than 0.01ng/ug cytokine as determined by the LAL assay.
Bioactivity: Measured by its ability to induce proliferation of B9 cells. The ED50 for this effect is typically 10 ng/mL.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-8219	Recombinant Human IL-21 (Interleukin-21, IL21)	N/A	N/A	BA ELISA std
34-8219	Carrier-Free Recombinant Human IL-21 (Interleukin-21, IL21)	N/A	N/A	BA ELISA std
39-8219	Single-Use ELISA RSG Standard Recombinant Human IL-21 (Interleukin-21, IL21)	N/A	N/A	ELISA std

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Description

Human Interleukin-21 (IL-21) is a 131-amino acid protein with 57% identity to the mouse gene. It contains a 24-amino acid signal peptide and a 4-helix-bundle cytokine domain homologous to IL-2, IL-4 and IL-15. IL-21 stimulates B cell proliferation in an anti-CD40 dependent manner but inhibits B cell proliferation stimulated by IL-4 plus anti-IgM. IL-21 is induced by IL-6 in activated T cells, a process that is dependent on STAT3 but not on ROR-gamma. IL-21 induces Th17 differentiation and suppresses FOXP3 expression, which requires STAT3 and ROR-gamma.

Applications Reported

For research use only, not for diagnostic or therapeutic use. For research use only, not for diagnostic or therapeutic use. The recombinant human IL-21 has been reported useful for bioassay and ELISA.

Applications Tested

This recombinant IL-21 has been tested in bioassay for its ability to induce proliferation of B9 cells. The ED50 for this effect is typically 10 ng/mL, corresponding to a specific activity of greater than or equal to 1.0 x 10E5 U/mg. The recombinant human IL-21 has been tested as the calibration standard in a human IL-21 sandwich ELISA using the ELISA pair eBio3A3-N2 / eBio2B2-G20, with a typical dynamic range of 4 ng/ml – 30 pg/ml).

References

Ettinger, R., et al., J. Immunol., 2005, 175: 7867 - 7879. IL-21 Induces Differentiation of Human Naive and Memory B Cells into Antibody-Secreting Plasma Cells. Ozaki, K., et al., J. Immunol., 2004; 173: 5361 - 5371. Regulation of B Cell Differentiation and Plasma Cell Generation by IL-21, a Novel Inducer of Blimp-1 and Bcl-6. Zeng, R., et al., Blood, 2007; 109: 4135 - 4142. The molecular basis of IL-21–mediated proliferation. Zeng, R., et al., J. Exp. Med., Jan 2005; 201: 139 - 148. Synergy of IL-21 and IL-15 in regulating CD8+ T cell expansion and function.

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