Contents: Carrier-Free Recombinant Human IL-17A (Interleukin-17A)
Catalog Number: 34-8179
Formulation: Sterile liquid; 35% (v/v) acetonitrile, 0.1% trifluoracetic acid (v/v) with no carrier or stabilizer. 0.22 uM filtered.
Storage Conditions: For greatest stability, keep concentration of primary stock at or above 10 µg/ml. For long term storage, aliquot into polypropylene vials (volumes of 20 µl or greater) and store at or below -80°C. Avoid repeated freeze/thaw cycles.
Handling Conditions: For best recovery, always quick-spin vial prior to opening. For dilution of current stock, always include carrier protein (1% BSA or 10% FBS) in the buffered saline diluent.
Source: E. coli expressed amino acids ile 20-ala 155 of mature human IL-17A (accession # NM_002190).
Molecular Mass: The methionylated polypeptide has a predicted molecular mass of 15,666. The DTT reduced protein migrates as a 15 kDa polypeptide. The protein is a cystine linked homodimer and migrates as a 27 kDa protein on non-reducing SDS-PAGE.
Purity: Greater than 98% as determined by SDS-PAGE
Endotoxin Level: Less than 0.01 ng/ug cytokine as determined by the LAL assay.
Bioactivity: Measured by induction of IL-6 production by NHDF cells. The ED50 is 3.0 ng/ml, corresponding to a specific activity of 3.3 x 105 Units/mg.
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Description
Interleukin-17A (IL-17A) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. The cDNA encoding human IL-17A was isolated from a library of CD4+ T cells; the encoded protein exhibits 72 percent amino acid identity with HVS13 , an open reading frame from a T lymphotropic Herpesvirus saimiri, and 63 percent with mouse CTLA-8 (cytotoxic T-lymphocyte associated antigen-8). Human IL-17A exists as glycosylated 20-30 kD homodimers.
High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells. IL-17A enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. Human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse receptor. IL-17A binds to a receptor that binds also to HVS13 (viral IL-17) and to CTLA-8.
Applications Reported
For research use only, not for diagnostic or therapeutic use. Recombinant human IL-17A is biologically active and can promote IL-6 production in vitro. The recombinant human IL-17A can be used as the standard for an IL-17A sandwich ELISA.
Applications Tested
The recombinant human IL-17A has been tested as the standard in a human IL-17A sandwich ELISA and in bioassay for induction of IL-6 production by NHDF cells. The ED
50 is 3.0 ng/ml, corresponding to a specific activity of 3.3 x 10
5 Units/mg.
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