Product Information

Contents: Carrier-Free Recombinant Human IL-17A/F (Interleukin-17A/F, IL17A/F)
Catalog Number: 34-8178
Sizes: 100 ug, 500 ug
Formulation: Sterile liquid; 20mM Sodium Phosphate, 0.2M NaCl, pH 6.5. 0.22 um filtered.
Storage Conditions: For greatest stability, keep concentration of primary stock at or above 10 ug/ml. For long term storage, aliquot and store at or below -80oC. Avoid repeated freeze/thaw cycles.
Handling Conditions: For best recovery, always quick-spin vial prior to opening. For dilution of current stock, always include carrier protein (1% BSA or 10% FBS) in the buffered saline diluent.
Source: Insect cell expressed human IL-17A disulfide-linked with IL-17F (accession # NM_002190, NM_052872).
Molecular Mass: The protein has a predicted molecular mass of 36,308. The non-reduced protein migrates as 35~36 kDa polypeptide on SDS-PAGE. The DTT reduced protein migrates as 22, 18 and 15 kDa polypeptides due to glycosylation. After PGNase F treatment, the DTT reduced protein migrates as 19 and 15 kDa polypeptides.
Purity: Greater than 98% as determined by SDS-PAGE.
Endotoxin Level: Less than 0.01ng/ug cytokine as determined by the LAL assay.
Bioactivity: Measured by induction of IL-6 production by NIH/3T3 cells. The ED50 is typically 10 ng/ml, corresponding to a specific activity of 1.0 x 10E5 Units/mg.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-8178	Recombinant Human IL-17A/F (Interleukin-17A/F, IL17A/F)	N/A	N/A	BA ELISA std
34-8178	Carrier-Free Recombinant Human IL-17A/F (Interleukin-17A/F, IL17A/F)	N/A	N/A	BA ELISA std

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Description

The interleukin 17 (IL-17) family proteins comprise six members (17A through 17F). However, recent studies found that co-expression of IL-17F and IL-17A in HEK293 cells results in the formation of biologically active IL-17F/IL-17A heterodimers, in addition to the IL-17F homodimers and IL-17A homodimers. Activated human CD4+ T cells were found to produce the IL-17A/F heterodimer, along with the corresponding homodimers. IL-17A was most potent, followed by IL-17A/F heterodimer, then IL-17F (100fold lower than IL-17A). Activated human CD4+ T cells in culture were found to secret IL-17F homodimer at 10-fold higher levels than IL-17A homodimer, suggesting that the majority of the IL-17A protein expressed exists in the form of the IL-17A/F heterodimer.

Applications Reported

For research use only, not for diagnostic or therapeutic use. For research use only, not for diagnostic or therapeutic use. The recombinant human IL-17A/F has been reported useful for bioassay and ELISA.

Applications Tested

This recombinant human IL-17A/F has been tested in bioassay for its ability to induce IL-6 production by NIH/3T3 cells. The ED50 for this effect is typically 10 ng/mL, corresponding to a specific activity of 1.0 x 10E5 U/mg.

References

Chang, S.H., Dong, C., 2007. A novel heterodimeric cytokine consisting of IL-17 and IL-17F regulates inflammatory responses. Cell Research 17:435-440. Wright, J.F., et al. 2007. Identification of an IL-17F/17A heterodimer in activated human CD4+ T cells. J. Biol. Chem. 282: 13447-13455

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