Mouse IL-15R/IL-15 Complex Recombinant Protein Carrier-Free
Also known as: Interleukin 15, IL15, IL-15 receptor, receptor/ligand complex
RUO: For Research Use Only
Proliferation of the CTLL-2 cell line in response to Mouse IL-15R/IL-15 Complex Recombinant Protein Carrier-Free
Contents: Mouse IL-15R/IL-15 Complex Recombinant Protein Carrier-Free Catalog Number: 34-8152 Formulation: Sterile liquid; 20mM Tris, 60mM NaCl, pH 8, 0.22 µm filtered. Storage Conditions: Store at less than or equal to -70°C. Handling Conditions: For best recovery, quick-spin vial prior to opening. Use in sterile envrioment. Source: Insect cell expressed N-terminal His-Tagged mIL-15Ra Gly33-Ala132 (accession # NP_032384) - GS linker - mIL-15 Asn49-Ser162 (accession # NP_032383). Molecular Mass: 29.4 kDa Purity: Greater than 98%, as determined by SDS-PAGE Endotoxin Level: Less than 0.1 ng/ug cytokine as determined by the LAL assay. Bioactivity: The ED50, tested in CTLL-2 cell proliferation assay, is 1 ng/ml, corresponding to a specific activity of approximately 106 Units/mg.
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34-8152-82
100 ug
34-8152-85
500 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
This recombinant protein is the heterocomplex of the mouse IL-15 linked to soluble IL-15 receptor alpha chain (sushi domain) via a GS linker.
Interleukin 15 (IL-15) is a cytokine that stimulates T and NK cell proliferation and differentiation. IL-15 regulates macrophage proinflammatory and chemotactic protein production. In addition, it induces differentiation of monocytes towards dendritic cells. The receptor for IL-15 is a high affinity heterotrimeric receptor composed of IL-15Rα and 2 additional subunits of the IL-2 receptor (beta and gamma). Upon activation, the receptor complex signals via the Stat and JAK pathways.
It has been reported that the soluble form of IL-15Rα corresponding to the entire extracellular domain, cleaved by MMP’s, behaves as a high affinity IL-15 antagonist, whereas the recombinant extracellular domain comprised of the sushi domain together with the ligand, IL-15, behaves as a potent agonist by enhancing binding of IL-15 to the receptor complex. In fact, this receptor-ligand complex has approximately 10-100 fold increased proliferative activity.
Applications Reported
For research use only, not for diagnostic or therapeutic use. Recombinant mouse IL-15/IL-15RA complex is biologically active.
Applications Tested
This recombinant mouse IL-15/IL-15RA complex has been tested in CTLL-2 cell proliferation assay with an observed ED50 of 1 ng/ml, corresponding to a specific activity of approximately 106 Units/mg.
References
Mortier E, Quéméner A, Vusio P, Lorenzen I, Boublik Y, Grötzinger J, Plet A, Jacques Y. Soluble interleukin-15 receptor alpha (IL-15R alpha)-sushi as a selective and potent agonist of IL-15 action through IL-15R beta/gamma. Hyperagonist IL-15 x IL-15R alpha fusion proteins. J Biol Chem. 2006 Jan 20;281(3):1612-9.
Teague, R. M. Sather, B. D. Sacks, J. A. Huang, M. Z. Dossett, M. L. Morimoto, J. Tan, X. Sutton, S. E. Cooke, M. P. Ohlen, C. Greenberg, P. D. Interleukin-15 rescues tolerant CD8+ T cells for use in adoptive immunotherapy of established tumors. Nature Med. 2006; 12: 335-341.
Vamosi G, Bodnar A, Vereb G, Jenei A, Goldman CK, Langowski J, Toth K, Matyus L, Szollosi J, Waldmann TA, Damjanovich S. IL-2 and IL-15 receptor alpha-subunits are coexpressed in a supramolecular receptor cluster in lipid rafts of T cells. Proc Natl Acad Sci U S A. 2004 Jul 27;101(30):11082-7.
Dubois S, Mariner J, Waldmann TA, Tagaya Y. IL-15Ralpha recycles and presents IL-15 In trans to neighboring cells. Immunity. 2002 Nov;17(5):537-47.
