Product Information

Contents: Carrier-Free Recombinant Mouse IL-12 (Interleukin-12) p70
Catalog Number: 34-8121
Sizes: 100 ug, 500 ug
Formulation: Sterile liquid; 10 mM NaH2PO4, pH 6.0, 300 mM NaCl, with no carrier or stabilizer. 0.22 uM filtered.
Storage Conditions: For greatest stability, keep concentration of primary stock at or above 10 µg/ml. For long term storage, aliquot into polypropylene vials (volumes of 20 µl or greater) and store at or below -80°C. Avoid repeated freeze/thaw cycles.
Handling Conditions: For best recovery, always quick-spin vial prior to opening. For dilution of current stock, always include carrier protein (1% BSA or 10% FBS) in the buffered saline diluent.
Source: Insect cells infected with baculovirus: mouse p40, amino acids met 1-ser 335, (accession # NM_008352) was co-expressed with mouse p35, amino acids met 1-ala 215, (accession # NM_008351).
Molecular Mass: The heterodimeric, p40, amino acids met 23-ser 335, cystine linked to p35, amino acids arg 23-ala 215, has a predicted molecular mass of 57,496. On non-reducing SDS-PAGE the heterodimeric cystine-linked protein migrates as a 65 kDa protein. The DTT reduced protein migrates as 43 kDa and 24 kDa polypeptides.
Purity: Greater than 98%, as determined by SDS-PAGE
Endotoxin Level: Less than 0.01 ng/ug cytokine, as determined by the LAL assay.
Bioactivity: Measured by assay of IFN-γ production by mouse splenocytes. The ED₅₀ is 0.3 ng/ml, corresponding to a specific activity of 3.0 x 10⁶ Units/mg.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-8121	Recombinant Mouse IL-12 (Interleukin-12, IL12) p70	N/A	N/A	BA ELISA std
34-8121	Carrier-Free Recombinant Mouse IL-12 (Interleukin-12, IL12) p70	N/A	N/A	BA ELISA
39-8121	Single-Use ELISA RSG Standard Recombinant Mouse IL-12 (Interleukin-12, IL12) p70	N/A	N/A	ELISA

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

Interleukin-12 (IL-12) is a heterodimeric 70 kD (p70) cytokine composed of two covalently linked, glycosylated chains, 40kD (p40) and 35-kD (p35). IL-12 is mainly produced by monocytes, macrophages, and dendritic cells in response to bacterial products such as lipopolysaccharides (LPS), to intracellular pathogens or upon interaction with activated T cells. IL-12 was originally discovered because of its ability to induce interferon-gamma (IFN-g) production, cell proliferation, and cytotoxicity mediated by natural killer cells and T cells. It is now established that IL-12 also plays a key role in the development of Th1 responses, leading to IFN-g and IL-2 production. These cytokines can in turn promote T-cell responses and macrophage activation.

eBioscience’s recombinant mouse IL-12 p70 is produced in baculovirus-infected insect cells as an authentic heterodimer of precursor p35 and p40 subunits using a dual promoter expression system. It is distinct from other available forms of the protein in that it is expressed as a true heterodimer, as opposed to a single-chain, pseudo-heterodimer in which the subunits are joined by an artificial linker.

Applications Reported

For research use only, not for diagnostic or therapeutic use. Recombinant mouse IL-12 p70 is biologically active and can be used as a standard for p40 or p70 ELISA.

Applications Tested

This recombinant mouse IL-12 has been tested by sandwich ELISA using the mouse IL-12 p40 ELISA (C15.6/C17.8) and the mouse IL-12 p70 ELISA (C18.2/C17.8).
This protein has been tested in bioassay for induction of IFN-γ production, yielding an ED₅₀ of less than 0.3 ng/ml, corresponding to a specific activity of approximately 3.0x10⁶ Units/mg.

References

Isler, P., et al. 1999. Am. J. Respir. Cell Mol. Biol. 20: 270-278.

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