Product Information

Contents: Carrier-Free Recombinant Mouse IL-7 (Interleukin-7)
Catalog Number: 34-8071
Sizes: 100 ug, 500 ug
Formulation: Sterile liquid; 35% (v/v) acetonitrile, 0.1% trifluoracetic acid (v/v) with no carrier or stabilizer. 0.22 uM filtered.
Storage Conditions: For greatest stability, keep concentration of primary stock at or above 10 µg/ml. For long term storage, aliquot into polypropylene vials (volumes of 20 µl or greater) and store at or below -80°C. Avoid repeated freeze/thaw cycles.
Handling Conditions: For best recovery, always quick-spin vial prior to opening. For dilution of current stock, always include carrier protein (1% BSA or 10% FBS) in the buffered saline diluent.
Source: E. coli expressed amino acids glu 26-ile 154 of mature mouse IL-7 (accession # NM_008371).
Molecular Mass: The methionylated polypeptide has a predicted molecular mass of 15,028. The DTT reduced protein migrates as a 15 kDa polypeptide. The non-reduced protein has slightly higher mobility on non-reducing SDS-PAGE.
Purity: Greater than 98% as determined by SDS-PAGE.
Endotoxin Level: Less than 0.01 ng/ug cytokine as determined by the LAL assay.
Bioactivity: Measured by mouse 2E8 cell proliferation assay. The ED₅₀ is 0.5 ng/ml, corresponding to a specific activity of 2.0 x 10⁶ Units/mg.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-8071	Recombinant Mouse IL-7 (Interleukin-7, IL7)	N/A	N/A	BA
34-8071	Carrier-Free Recombinant Mouse IL-7 (Interleukin-7, IL7)	N/A	N/A	BA

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Description

Mouse IL-7 (also called Lymphopoietin 1, LP-1), a pre-B cell growth factor, is an ~25 kDa factor produced by bone marrow and thymic stromal cells, and spleen cells. IL-7 is a stromal cell-derived growth factor for progenitor B cells and T cells. The main population in the thymus responsive to IL-7 is CD4^-CD8^-. It also stimulates proliferation and differentiation of mature T cells and NK cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. Recombinant mouse IL-7 is biologically active.

Applications Tested

This recombinant mouse IL-7 has been tested in bioassays with an observed ED₅₀ of approximately 500 pg/ml, corresponding to a specific activity of approximately 2x10⁶ Units/mg.