Contents: Carrier-Free Recombinant Mouse IL-5 (Interleukin-5)
Catalog Number: 34-8051
Formulation: Sterile liquid; 10 mM sodium phosphate, pH 7.2, 150 mM NaCl, with no carriers or preservatives. 0.22 µm filtered.
Storage Conditions: For greatest stability, keep concentration of primary stock at or above 10 µg/ml. For long term storage, aliquot into polypropylene vials (volumes of 20 µl or greater) and store at or below -80°C. Avoid repeated freeze/thaw cycles.
Handling Conditions: For best recovery, always quick-spin vial prior to opening. For dilution of current stock, always include carrier protein (1% BSA or 10% FBS) in the buffered saline diluent.
Source: Insect cells infected with baculovirus: amino acids Met 21- Gly 133 of mouse IL-5 (accession # NM_010558).
Molecular Mass: The protein has a predicted molecular mass of 13,126. The DTT reduced protein migrates as two major bands of 16 and 21 kDa and the non-reduced protein migrates as a group of bands corresponding to dimer at 37-39 kDa. Heterogeneity results from different glyscosylation states.
Purity: Greater than 98%, as determined by SDS-PAGE
Endotoxin Level: Less than 0.01 ng/ug cytokine as determined by the LAL assay.
Bioactivity: Measured by human TF-1 cell proliferation assay. The ED50 is 0.1 ng/ml, corresponding to a specific activity of 1.0 x 10E7 Units/mg.
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Description
Recombinant mouse IL-5 is a disulfide-linked homodimer, containing two 113 amino acid peptides; these resolve as 32-34 kDa bands in SDS-PAGE. IL-5 is produced by T cells and has been known as eosinophil-differentiating factor (EDF), B cell growth factor II (BCGFII), and T cell-replacing factor (TRF). IL-5 induces eosinophil differentiation and promotes eosinophil survival and activation. In mice, IL-5 has been shown to stimulate B cell proliferation and antibody production.
Applications Reported
For research use only, not for diagnostic or therapeutic use. Recombinant mouse IL-5 is biologically active and is useful for bioassays and as a cytokine standard in mouse IL-5 ELISA
Applications Tested
This recombinant mouse IL-5 has been tested by sandwich ELISA using the anti-mouse IL-5 antibody pair (TRFK5/TRFK4), and in bioassays using the IL-5 responsive human cell line TF-1. The ED
50 measured in a TF-1 proliferation assay is typically 100 pg/ml, corresponding to a specific activity of approximately 1x10
7 Units/mg.
