Product Information

Contents: Carrier-Free Recombinant Mouse IL-4 (Interleukin-4)
Catalog Number: 34-8041
Sizes: 100 ug, 500 ug
Formulation: Sterile liquid; phosphate buffered saline, pH 7.2, 150 mM NaCl, with no carriers or perservatives. 0.22 µm filtered.
Storage Conditions: For greatest stability, keep concentration of primary stock at or above 10 µg/ml. For long term storage, aliquot into polypropylene vials (volumes of 20 µl or greater) and store at or below -80°C. Avoid repeated freeze/thaw cycles.
Handling Conditions: For best recovery, always quick-spin vial prior to opening. For dilution of current stock, always include carrier protein (1% BSA or 10% FBS) in the buffered saline diluent.
Source: E. coli expressed amino acids his 21-ser140 of mature mouse IL-4 (accession # NM_021283).
Molecular Mass: The protein is not methionylated at the N-terminal and has a predicted molecular mass of 13,557. The DTT reduced protein migrates as a 13 kDa polypeptide on SDS-PAGE.
Purity: Greater than 98%, as determined by SDS-PAGE
Endotoxin Level: Less than 0.01 ng/ug cytokine as determined by the LAL assay.
Bioactivity: Measured by mouse CTLL2 cell proliferation assay. The ED₅₀ is 0.1 ng/ml, corresponding to a specific activity of 1.0 x 10⁷ Units/mg.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-8041	Recombinant Mouse IL-4 (Interleukin-4, IL4)	N/A	N/A	BA ELISA
34-8041	Carrier-Free Recombinant Mouse IL-4 (Interleukin-4, IL4)	N/A	N/A	BA ELISA
39-8041	Single-Use ELISA RSG Standard Recombinant Mouse IL-4 (Interleukin-4, IL4)	N/A	N/A	ELISA

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

Mouse IL-4, also known as BCDF, BCGF-1 and BSF-1, is secreted by activated Th2 and natural killer T cells, and to a lesser extent by Th1 and mast cells. IL-4 is species-specific and promotes proliferation and differentiation of B cells. IL-4, in conjunction with other signals such as CD40, induces Ig isotype switching of B cells and the upregulation of surface MHC class II and CD23.

Applications Reported

For research use only, not for diagnostic or therapeutic use. Recombinant mouse IL-4 is biologically active and can promote proliferation of mouse T lymphocytes in culture. It is also used as a cytokine standard in mouse IL-4 ELISA.

Applications Tested

The recombinant mouse IL-4 has been tested by sandwich ELISA using the anti-mouse IL-4 antibody pair (11B11/BVD6-24G2) and in bioassays using the mouse cell line CTLL-2. The ED₅₀ measured in a CTLL-2 proliferation assay is typically 100 pg/ml, corresponding to a specific activity of approximately 1x10⁷ Units/mg.

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