mouse IL-3 (Interleukin-3)

Contents: Carrier-Free Recombinant Mouse IL-3 (Interleukin-3)
Catalog Number: 34-8031
Formulation: Sterile liquid; phosphate buffered saline, pH 7.2, 150 mM NaCl, with 1.0% BSA. 0.22 µm filtered.
Storage Conditions: For greatest stability, keep concentration of primary stock at or above 10 µg/ml. For long term storage, aliquot into polypropylene vials (volumes of 20 µl or greater) and store at or below -80°C. Avoid repeated freeze/thaw cycles.
Handling Conditions: For best recovery, always quick-spin vial prior to opening. For dilution of current stock, always include carrier protein (1% BSA or 10% FBS) in the buffered saline diluent.
Source: E. coli expressed amino acids ala 27-cys 166 of mature mouse IL-3 (accession # NM_010556).
Molecular Mass: The protein may be methionylated at the N-terminal and without the met has a predicted molecular mass of 15,673. The DTT reduced protein migrates as an 16 kDa polypeptide on SDS-PAGE. The non-reduced protein migrates as a 14 kDa polypeptide.
Purity: Greater than 98%, as determined by SDS-PAGE
Endotoxin Level: Less than 0.01 ng/ug cytokine as determined by the LAL assay.
Bioactivity: Measured by mouse MC/9 cell proliferation assay. The ED₅₀ is 0.1 ng/ml, corresponding to a specific activity of 1.0 x 10⁷ Units/mg.

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
34-8031-82	100 ug	$1200
34-8031-85	500 ug	$3120

*International customers: Please contact your distributor for region specific pricing.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-8031	Recombinant Mouse IL-3 (Interleukin-3, IL3)	N/A	N/A	BA
34-8031	Carrier-Free Recombinant Mouse IL-3 (Interleukin-3, IL3)	N/A	N/A	BA

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

Mouse IL-3, also called Mast Cell Growth Factor (MCGF), Multi-Colony Stimulating Factor (multi-CSF), Eosinophil-CSF (E-CSF), is produced by activated T cells, mast cells and eosinophils. IL-3 is a hematopoietic growth factor that stimulates colony formation of erythroid, megakaryocyte, neutrophil, eosinophil, basophil, mast cell and monocytic lineages. Most of these functions are enhanced or dependent on co-stimulation with other cytokines.

Applications Reported

For research use only, not for diagnostic or therapeutic use. Recombinant mouse IL-3 is biologically active.

Applications Tested

This recombinant mouse IL-3 has been tested by MC/9 cell proliferation bioassay with an observed ED₅₀ of 100 pg/ml, corresponding to a specific activity of approximately 1x10⁷ Units/mg.

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