Product Information

Contents: Carrier-Free Recombinant Human IL-1a (Interleukin-1 alpha, IL1a)
Catalog Number: 34-8019
Sizes: 100 ug, 500 ug
Formulation: Sterile liquid; 20 mM Tris pH 8.0, 4 mM DTT with no carriers or stabilizers. 0.22 µm filtered.
Storage Conditions: For greatest stability, keep concentration of primary stock at or above 10 µg/ml. For long term storage, aliquot into polypropylene vials (volumes of 20 µl or greater) and store at or below -20°C in a non-cycling freezer (e.g., not ‘frost free’). Avoid repeated freeze/thaw cycles.
Handling Conditions: For best recovery, always quick-spin vial prior to opening. For dilution of current stock, always include carrier protein (1% BSA or 10% FBS) in the buffered saline diluent.
Source: E. coli
Purity: Greater than 98%, as determined by SDS-PAGE
Endotoxin Level: Less than 0.01 ng/ug cytokine as determined by the LAL assay.
Bioactivity: Measured by mouse D10S cell proliferation assay. The ED₅₀ is 20.0 pg/ml, corresponding to a specific activity of 5.0 x 10⁷ U/mg.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-8019	Recombinant Human IL-1a (Interleukin-1 alpha, IL1a)	N/A	N/A	BA ELISA
34-8019	Carrier-Free Recombinant Human IL-1a (Interleukin-1 alpha, IL1a)	N/A	N/A	BA

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Description

Human IL-1α, also called lymphocyte activating factor (LAF), endogenous pyrogen (EP), leucocyte endogenous mediator (LEM), and mononuclear cell factor (MCF), is produced by a wide variety of cells, including macrophages, dendritic cells, T cells and B cells. IL-1α is mostly cell associated and has only 20% amino acid homology with IL-1β. The immune regulatory role of IL-1α is exerted on a wide range of cells including lymphocytes, epithelial cells and fibroblasts. In vivo, it induces hypotension, fever, and acute phase response.

Applications Reported

For research use only, not for diagnostic or therapeutic use. Recombinant human IL-1α is biologically active and is also useful as a cytokine standard for sandwich ELISA.

Applications Tested

The recombinant human IL-1α has been tested as the standard in a human IL-1α sandwich ELISA using the human IL-1α ELISA pair (CRM8/CRM6), and in D10 cell proliferation bioassay, with an observed ED₅₀ of 20 pg/ml, corresponding to a specific activity of approximately 5x10⁷ Units/mg.