Product Information

Contents: Carrier-Free Recombinant Mouse IL-1b (Interleukin-1 beta)
Catalog Number: 34-8012
Sizes: 100 ug, 500 ug
Formulation: Sterile liquid; 10 mM sodium phosphate, pH 7.2, 150 mM NaCl, with no carriers or preservatives. 0.22 µm filtered.
Storage Conditions: For greatest stability, keep concentration of primary stock at or above 10 µg/ml. For long term storage, aliquot into polypropylene vials (volumes of 20 µl or greater) and store at or below -80°C. Avoid repeated freeze/thaw cycles.
Handling Conditions: For best recovery, always quick-spin vial prior to opening. For dilution of current stock, always include carrier protein (1% BSA or 10% FBS) in the buffered saline diluent.
Source: E. coli expressed amino acids Val 118-Ser 269 of mouse IL-1β (accession # P10749).
Molecular Mass: The protein is not methionylated at the N-terminal and has a predicted molecular mass of 17,393. The DTT reduced and non-reduced proteins migrate as 18 kDa polypeptides on SDS-PAGE.
Purity: Greater than 98%, as determined by SDS-PAGE
Endotoxin Level: Less than 0.01 ng/ug cytokine as determined by the LAL assay.
Bioactivity: Measured by mouse D10S cell proliferation assay. The ED50 is 0.01 ng/ml, corresponding to a specific activity of 1.0 x 10E8 U/mg.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-8012	Recombinant Mouse IL-1b (Interleukin-1 beta, IL1b)	N/A	N/A	BA ELISA
34-8012	Carrier-Free Recombinant Mouse IL-1b (Interleukin-1 beta, IL1b)	N/A	N/A	BA ELISA
39-8012	Single-Use ELISA RSG Standard Recombinant Mouse IL-1b (Interleukin-1 beta, IL1b)	N/A	N/A	ELISA

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

Mouse IL-1β, also called lymphocyte activating factor (LAF), endogenous pyrogen (EP), leucocyte endogenous mediator (LEM), mononuclear cell factor (MCF), is a ~17 kDa factor produced by a wide variety of cells, including macrophages, dendritic cells, T and B cells. IL-1β is mostly cell associated with 23% amino acid homology with IL-1α. The immune regulatory role of IL-1β is exerted on a wide range of cells including lymphocytes, epithelial cells and fibroblasts. In vivo, it induces hypotension, fever, and acute phase response.

Applications Reported

For research use only, not for diagnostic or therapeutic use. Recombinant mouse IL-1β has been reported for use in cytokine bioassays. This recombinant protein is biologically active.

Applications Tested

This reagent has been tested by sandwich ELISA using the anti-mouse IL-1β ELISA pair (B122/biotin-polyclonal) and in bioassays, with an observed ED₅₀ of 10 pg/ml, corresponding to a specific activity of approximately 1x10⁸ Units/mg.