Product Information

Contents: Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-human HLA-DR
Catalog Number: 27-9956
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures. This product is guaranteed for 6 months upon receipt when stored properly.
Clone: LN3
Isotype: Mouse IgG2b, κ

Staining of normal human peripheral blood cells with 0.125 μg of APC-Alexa Fluor® 750 Mouse IgG2b Iso Cntrl (cat. 27-4732) (open histogram) or APC-Alexa Fluor® 750 anti-human HLA-DR(LN3) (colored histogram). Cells in the lymphocyte (left) and monocyte (right) gates were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9956	Affinity Purified anti-human HLA-DR	N/A	N/A	FC IHC
13-9956	Biotin anti-human HLA-DR	N/A	N/A	FC
11-9956	Fluorescein isothiocyanate (FITC) anti-human HLA-DR	488	518	FC
12-9956	PE anti-human HLA-DR	488	575	FC
15-9956	Phycoerythrin-Cy5 (PE-Cy5) anti-human HLA-DR	488	670	FC
17-9956	APC anti-human HLA-DR	633	660	FC
25-9956	Phycoerythrin-Cy7 (PE-Cy7) anti-human HLA-DR	488	760	FC
56-9956	Alexa Fluor® 700 anti-human HLA-DR (APC-Cy5.5 Equivalent)	633	723	FC
27-9956	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-human HLA-DR	633	779	FC
45-9956	PerCP-Cy5.5 anti-human HLA-DR (PE-Cy5.5 replacement)	488	690	FC
93-9956	eFluor^TM 605^NC anti-human HLA-DR	350-500	605	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The LN3 mAb reacts with the human major histocompatibility complex (MHC) class II, HLA-DR. HLA-DR is expressed on the surface of human antigen presenting cells (APC) including B cells, monocytes, macrophages, DCs, and activated T cells. HLA-DR is a heterodimeric transmembrane protein composed of α and β subunits and plays an important role in the presentation of peptides to CD4⁺ T lymphocytes.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This LN3 antibody has been reported for use in flow cytometric analysis.

The photo-emission properties and compensation required for Alexa Fluor® 750 are similar to APC-Cy7; however Alexa Fluor® 750 has several advantages when compared to APC-Cy7. The Alexa Fluor® 750 has peak emission of 779 nm, and usually has a slightly higher mean fluorescent intensity when compared to the same clone conjugated with APC-Cy7, which has peak emission of 767 nm. Furthermore, Alexa Fluor® 750 is considered to be a more photostable fluorochrome as compared with APC-Cy7. It is advised not to use fixatives with Alexa Fluor® 750 as this may lead to degradation of the fluorochrome and loss of staining.

Applications Tested

This LN3 antibody has been pre-titrated and tested by flow cytometric analysis of normal normal human peripheral blood cells. This can be used at 20 μl (0.03 μg) per million cells in a 100 μl total staining volume.

References

Fullen, D. R. and J. T. Headington (1998). "Factor XIIIa-positive dermal dendritic cells and HLA-DR expression in radial versus vertical growth-phase melanomas [see comments]." J Cutan Pathol 25(10): 553-8.
Hua, Z. X., K. E. Tanaka, et al. (1998). "Immunoreactivity for LN2 and LN3 distinguishes small cell carcinomas from non-small cell carcinomas in the lung." Hum Pathol 29(12): 1441-6.
Ioachim, H. L., S. E. Pambuccian, et al. (1996). "Lymphoid monoclonal antibodies reactive with lung tumors. Diagnostic applications." Am J Surg Pathol 20(1): 64-71.
Davey, F. R., S. Olson, et al. (1988). "The immunophenotyping of extramedullary myeloid cell tumors in paraffin-embedded tissue sections." Am J Surg Pathol 12(9): 699-707.
Norton, A. J. and P. G. Isaacson (1987). "Detailed phenotypic analysis of B-cell lymphoma using a panel of antibodies reactive in routinely fixed wax-embedded tissue." Am J Pathol 128(2): 225-40.

Related Products

Cat. 27-4732 Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) Mouse IgG2b Isotype Control (APC-Cy7 Equivalent)

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.