Product Information

Contents: Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-mouse TCR beta (TCRb)
Catalog Number: 27-5961
Sizes: 50 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures. This product is guaranteed for 6 months upon receipt when stored properly.
Clone: H57-597
Isotype: Armenian Hamster IgG

Staining of BALB/c splenocytes with APC anti-mo/hu B220 (RA3-6B2) (cat. 17-0452) and 0.5 μg of APC-Alexa Fluor® 750 Armenian Hamster IgG Iso Cntrl (cat. 27-4888) (left) or 0.5 μg of APC-Alexa Fluor® 750 anti-mouse TCRb (H57-597) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5961	Affinity Purified anti-mouse TCR beta (TCRb)	N/A	N/A	FA FC IHC
16-5961	Functional Grade* Purified anti-mouse TCR beta (TCRb)	N/A	N/A	FA FC
13-5961	Biotin anti-mouse TCR beta (TCRb)	N/A	N/A	FC
11-5961	FITC anti-mouse TCR beta (TCRb)	488	518	FC
12-5961	PE anti-mouse TCR beta (TCRb)	488	575	FC
15-5961	PE-Cy5 anti-mouse TCR beta (TCRb)	488	670	FC
17-5961	APC anti-mouse TCR beta (TCRb)	633	660	FC
35-5961	Phycoerythrin-Cy5.5 (PE-Cy5.5) anti-mouse TCR beta (TCRb)	488	690	FC
36-5961	Functional Grade* Biotin anti-mouse TCR beta (TCRb)	N/A	N/A	FA FC IHC
27-5961	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-mouse TCR beta (TCRb) (APC-Cy7 Equivalent)	633	779	FC
45-5961	PerCP-Cy5.5 anti-mouse TCR beta (TCRb)	488	690	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The H57-597 monoclonal antibody reacts with the β chain of mouse TCR. TCRβ is expressed by thymocytes in a developmentally regulated manner and a majority of peripheral T cells. Crosslinking of the TCR complex with H57-597 induces activation and proliferation of T cells or apoptosis based on assay conditions. H57-597 is used as a phenotypic marker for TCR β expressing T cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This H57-597 antibody has been reported for use in flow cytometric analysis.

The photo-emission properties and compensation required for Alexa Fluor® 750 are similar to APC-Cy7; however Alexa Fluor® 750 has several advantages when compared to APC-Cy7. The Alexa Fluor® 750 has peak emission of 779 nm, and usually has a slightly higher mean fluorescent intensity when compared to the same clone conjugated with APC-Cy7, which has peak emission of 767 nm. Furthermore, Alexa Fluor® 750 is considered to be a more photostable fluorochrome as compared with APC-Cy7. It is advised not to use fixatives with Alexa Fluor® 750 as this may lead to degradation of the fluorochrome and loss of staining.

Applications Tested

This H57-597 antibody has been tested by flow cytometric analysis of mouse splenocytes and thymocytes. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Kubo, R. T., W. Born, et al. (1989). "Characterization of a monoclonal antibody which detects all murine alpha beta T cell receptors." J Immunol 142(8): 2736-42.

Related Products

Cat. 27-4888 Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) Armenian Hamster IgG Isotype Control (APC-Cy7 Equivalent) (clone eBio299Arm)

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.