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Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq. DescriptionThe RB6-8C5 monoclonal antibody reacts with mouse Ly-6G and Ly-6C, a 21-25 kDa protein also known as the myeloid differentiation antigen Gr-1. A GPI-linked protein, Gr-1 is expressed by the myeloid lineage in a developmentally regulated manner in the bone marrow. While monocytes only express Gr-1 transiently during their bone marrow development, the expression of Gr-1 on bone marrow granulocytes as well as on peripheral neutrophils is a good marker for these populations. Applications ReportedFor research use only, not for diagnostic or therapeutic use. This RB6-8C5 antibody has been reported for use in flow cytometric analysis. The photo-emission properties and compensation required for Alexa FluorŽ 750 are similar to APC-Cy7; however Alexa FluorŽ 750 has several advantages when compared to APC-Cy7. The Alexa FluorŽ 750 has peak emission of 779 nm, and usually has a slightly higher mean fluorescent intensity when compared to the same clone conjugated with APC-Cy7, which has peak emission of 767 nm. Furthermore, Alexa FluorŽ 750 is considered to be a more photostable fluorochrome as compared with APC-Cy7. It is advised not to use fixatives with Alexa FluorŽ 750 as this may lead to degradation of the fluorochrome and loss of staining. Applications TestedThis RB6-8C5 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and splenocyte suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. References
Hestdal, K., F. W. Ruscetti, et al. (1991). "Characterization and regulation of RB6-8C5 antigen expression on murine bone marrow cells." J Immunol 147(1): 22-8. Fleming, T. J., M. L. Fleming, et al. (1993). "Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family." J Immunol 151(5): 2399-408.(RB6-8C5; Cross-reactivity to Ly-6G and Ly-6C; PubMed) Sasmono, R.T., A. Ehrnsperger, et al. (2007). "Mouse neutrophilic granulocytes express mRNA encoding the macrophage colony-stimulating factor receptor (CSF-1R) as well as many other macrophage-specific transcripts and can transdifferentiate into macrophages in vitro in response to CSF-1." J Leukocyte Biol 82: 111-23.(RB6-8C5; Cross-reactivity to Ly-6G and Ly-6C; Pubmed) Related ProductsCat. 27-4331 Allophycocyanin-Alexa FluorŽ 750 (APC-Alexa FluorŽ 750) Rat IgG2b Isotype Control (APC-Cy7 Equivalent) Alexa FluorŽ and Pacific BlueŽ are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc. |
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Copyright Š 2000-2008 eBioscience, Inc. Product For Research Use Only: Not for further distribution without written consent. |
Mouse Cellular Antigens Chart
