Product Information

Contents: Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-mouse TER-119 (TER119, Erythroid cells, Ly-76)
Catalog Number: 27-5921
Sizes: 50 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures. This product is guaranteed for 6 months upon receipt when stored properly.
Clone: TER-119
Isotype: Rat IgG2b, κ

Staining of C57Bl/6 splenocytes with APC anti-mouse CD45 (30-F11) (cat. 17-0451) and 0.25 μg of APC-Alexa Fluor® 750 Rat IgG2b Iso Cntrl (cat. 27-4331) (left) or 0.25 μg of APC-Alexa Fluor® 750 anti-mouse Erythroid cells (TER-119) (right). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-5921	FITC anti-mouse TER-119 (TER119, Erythroid cells, Ly-76)	488	518	FC
12-5921	PE anti-mouse TER-119 (TER119, Erythroid cells, Ly-76)	488	575	FC
13-5921	Biotin anti-mouse TER-119 (TER119, Erythroid cells, Ly-76)	N/A	N/A	FC
14-5921	Affinity Purified anti-mouse TER-119 (TER119, Erythroid cells, Ly-76)	N/A	N/A	FC IHC IP WB
15-5921	PE-Cy5 anti-mouse TER-119 (TER119, Erythroid cells, Ly-76)	488	670	FC
16-5921	Functional Grade* Purified anti-mouse TER-119 (TER119, Erythroid cells, Ly-76)	N/A	N/A	FC
17-5921	APC anti-mouse TER-119 (TER119, Erythroid cells, Ly-76)	633	660	FC
25-5921	PE-Cy7 anti-mouse TER-119 (TER119, Erythroid cells, Ly-76)	488	760	FC
27-5921	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-mouse TER-119 (TER119, Erythroid cells, Ly-76) (APC-Cy7 Equivalent)	633	779	FC
35-5921	Phycoerythrin-Cy5.5 (PE-Cy5.5) anti-mouse TER-119 (TER119, Erythroid cells, Ly-76)	488	690	FC
36-5921	Functional Grade* Biotin anti-mouse TER-119 (TER119, Erythroid cells, Ly-76)	N/A	N/A	FC
45-5921	PerCP-Cy5.5 anti-mouse TER-119 (TER119, Erythroid cells, Ly-76)	488	690	FC
57-5921	Pacific Blue® anti-mouse TER-119 (TER119, Erythroid cells, Ly-76)	402	421	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The TER-119 monoclonal antibody reacts with mouse erythroid cells from early proerythroblast to mature erythrocyte stages. The TER-119 antigen is present in yolk sac, fetal and newborn liver, but is not expressed by cells carrying BFU-E and CFU-E activities. Several erythroleukemia cell lines tested so far are negative for expression of TER-119 antigen even after dimethylsulfoxide stimulation. Biochemical and molecular analysis of the TER-119 antigen indicate that this molecule is associated with the surface glycophorin A, but is not a typical glycophorin.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This TER-119 antibody has been reported for use in flow cytometric analysis.

The photo-emission properties and compensation required for Alexa Fluor® 750 are similar to APC-Cy7; however Alexa Fluor® 750 has several advantages when compared to APC-Cy7. The Alexa Fluor® 750 has peak emission of 779 nm, and usually has a slightly higher mean fluorescent intensity when compared to the same clone conjugated with APC-Cy7, which has peak emission of 767 nm. Furthermore, Alexa Fluor® 750 is considered to be a more photostable fluorochrome as compared with APC-Cy7. It is advised not to use fixatives with Alexa Fluor® 750 as this may lead to degradation of the fluorochrome and loss of staining.

Applications Tested

This TER-119 antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow cell suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Kina, T., K. Ikuta, et al. (2000). "The monoclonal antibody TER-119 recognizes a molecule associated with glycophorin A and specifically marks the late stages of murine erythroid lineage." Br J Haematol 109(2): 280-87.
Vannucchi, A. M., F. Paoletti, et al. (2000). "Identification and characterization of a bipotent (erythroid and megakaryocytic) cell precursor from the spleen of phenylhydrazine-treated mice." Blood 95(8): 2559-68.

Related Products

Cat. 27-4031 Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) Rat IgG2b Isotype Control (APC-Cy7 Equivalent)

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.