Product Information

Contents: Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) Armenian Hamster IgG Isotype Control
Catalog Number: 27-4888
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures. This product is guaranteed for 6 months upon receipt when stored properly.
Clone: eBio299Arm
Isotype: Armenian Hamster IgG

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-4888	Affinity Purified Armenian Hamster IgG Isotype Control	N/A	N/A	FC
16-4888	Functional Grade* Purified Armenian Hamster IgG Isotype Control	N/A	N/A	FC
13-4888	Biotin Armenian Hamster IgG Isotype Control	N/A	N/A	FC
11-4888	Fluorescein isothiocyanate (FITC) Armenian Hamster IgG Isotype Control	488	518	FC
12-4888	Phycoerythrin (PE) Armenian Hamster IgG Isotype Control	488	575	FC
15-4888	Phycoerythrin-Cy5 (PE-Cy5) Armenian Hamster IgG Isotype Control	488	670	FC
17-4888	Allophycocyanin (APC) Armenian Hamster IgG Isotype Control	633	660	FC
25-4888	Phycoerythrin-Cy7 (PE-Cy7) Armenian Hamster IgG Isotype Control	488	760	FC
35-4888	Phycoerythrin-Cy5.5 (PE-Cy5.5) Armenian Hamster IgG Isotype Control	488	690	FC
51-4888	Alexa Fluor® 647 Armenian Hamster IgG Isotype Control	633	668	FC
53-4888	Alexa Fluor® 488 Armenian Hamster IgG Isotype Control	488	519	FC
56-4888	Alexa Fluor® 700 Armenian Hamster IgG Isotype Control (APC-Cy5.5 Equivalent)	633	723	FC
45-4888	PerCP-Cy5.5 Armenian Hamster IgG Isotype Control	488	690	FC
93-4888	eFluor^TM 605^NC Armenian Hamster IgG Isotype Control	350-500	605	FC
94-4888	eFluor^TM 625^NC Armenian Hamster IgG Isotype Control	350-500	625	FC
47-4888	Allophycocyanin-eFluor^TM 780 Armenian Hamster IgG Isotype Control (APC-Alexa Fluor® 750 replacement)	633	780	FC
48-4888	eFluor^TM 450 Armenian Hamster IgG Isotype Control (Pacific Blue® replacement)	405	450	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eBio299Arm monoclonal antibody is useful as an isotype control immunoglobulin.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBio299Arm antibody has been reported for use in flow cytometric analysis.

The photo-emission properties and compensation required for Alexa Fluor® 750 are similar to APC-Cy7; however Alexa Fluor® 750 has several advantages when compared to APC-Cy7. The Alexa Fluor® 750 has peak emission of 779 nm, and usually has a slightly higher mean fluorescent intensity when compared to the same clone conjugated with APC-Cy7, which has peak emission of 767 nm. Furthermore, Alexa Fluor® 750 is considered to be a more photostable fluorochrome as compared with APC-Cy7. It is advised not to use fixatives with Alexa Fluor® 750 as this may lead to degradation of the fluorochrome and loss of staining.

Applications Tested

This eBio299Arm antibody has been tested by flow cytometric analysis and should be used at the same concentration as the experimental antibody.

Related Products

Cat. 27-4888 Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) Armenian Hamster IgG Isotype Control (APC-Cy7 Equivalent) (clone eBio299Arm)
Cat. 27-4914 Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) Golden Syrian Hamster IgG Isotype Control (APC-Cy7 Equivalent) (clone n/a)

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.