Product Information

Contents: Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) Mouse IgG2a, K Isotype Control
Catalog Number: 27-4724
Sizes: 50 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
500 mM NaCl, 0.09% NaN₃, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures. This product is guaranteed for 6 months upon receipt when stored properly.
Isotype: Mouse IgG2a, κ

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-4724	Fluorescein isothiocyanate (FITC) Mouse IgG2a, K Isotype Control	488	518	FC
12-4724	Phycoerythrin (PE) Mouse IgG2a, K Isotype Control	488	575	FC
15-4724	Phycoerythrin-Cy5 (PE-Cy5) Mouse IgG2a, K Isotype Control	488	670	FC
17-4724	Allophycocyanin (APC) Mouse IgG2a, K Isotype Control	633	660	FC
25-4724	Phycoerythrin-Cy7 (PE-Cy7) Mouse IgG2a, K Isotype Control	488	760	FC
30-4724	DISCONTINUED - Allophycocyanin-Cy5.5 (APC-Cy5.5) Mouse IgG2a, K Isotype Control (Alexa Fluor® 700 Equivalent)	633	690	FC
35-4724	PE-Cy5.5 Mouse IgG2a, K Isotype Control	488	690	FC
51-4724	Alexa Fluor® 647 Mouse IgG2a, K Isotype Control	633	668	FC
53-4724	Alexa Fluor® 488 Mouse IgG2a, K Isotype Control	488	519	FC
56-4724	Alexa Fluor® 700 Mouse IgG2a, K Isotype Control (APC-Cy5.5 Equivalent)	633	723	FC
57-4724	Pacific Blue® Mouse IgG2a, K Isotype Control	402	421	FC
27-4724	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) Mouse IgG2a, K Isotype Control (APC-Cy7 Equivalent)	633	779	FC
45-4724	PerCP-Cy5.5 Mouse IgG2a, K Isotype Control	488	690	FC
93-4724	eFluor^TM 605^NC Mouse IgG2a, Isotype Control	350-500	605	FC
94-4724	eFluor^TM 625^NC Mouse IgG2a Isotype Control	350-500	625	FC
14-4724	Affinity Purified Mouse IgG2a, K Isotype Control	N/A	N/A	FC
16-4724	Functional Grade* Purified Mouse IgG2a, K Isotype Control	N/A	N/A	FC
13-4724	Biotin Mouse IgG2a, K Isotype Control	N/A	N/A	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

This is a monoclonal mouse IgG2a, κ antibody. It is used as an isotype control for mouse IgG2a antibodies.

Applications Reported

For research use only, not for diagnostic or therapeutic use. APC-Alexa Fluor® 750 Mouse IgG2a, K Iso Cntrl has been reported for use in flow cytometric analysis.

The photo-emission properties and compensation required for Alexa Fluor® 750 are similar to APC-Cy7; however Alexa Fluor® 750 has several advantages when compared to APC-Cy7. The Alexa Fluor® 750 has peak emission of 779 nm, and usually has a slightly higher mean fluorescent intensity when compared to the same clone conjugated with APC-Cy7, which has peak emission of 767 nm. Furthermore, Alexa Fluor® 750 is considered to be a more photostable fluorochrome as compared with APC-Cy7. It is advised not to use fixatives with Alexa Fluor® 750 as this may lead to degradation of the fluorochrome and loss of staining.

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.