Product Information

Contents: Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) Mouse IgG1, K Isotype Control
Catalog Number: 27-4714
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
500 mM NaCl, 0.09% NaN₃, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures. This product is guaranteed for 6 months upon receipt when stored properly.
Isotype: Mouse IgG1, κ

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-4714	Fluorescein isothiocyanate (FITC) Mouse IgG1, K Isotype Control	488	518	FC
12-4714	Phycoerythrin (PE) Mouse IgG1, K Isotype Control	488	575	FC
13-4714	Biotin Mouse IgG1, K Isotype Control	N/A	N/A	FC
14-4714	Affinity Purified Mouse IgG1, K Isotype Control	N/A	N/A	FC IC Flow IP WB
15-4714	Phycoerythrin-Cy5 (PE-Cy5) Mouse IgG1, K Isotype Control	488	670	FC
16-4714	Functional Grade* Purified Mouse IgG1, K Isotype Control	N/A	N/A	FC
17-4714	Allophycocyanin (APC) Mouse IgG1, K Isotype Control	633	660	FC
25-4714	Phycoerythrin-Cy7 (PE-Cy7) Mouse IgG1, K Isotype Control	488	760	FC
27-4714	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) Mouse IgG1, K Isotype Control (APC-Cy7 Equivalent)	633	779	FC
30-4714	DISCONTINUED - Allophycocyanin-Cy5.5 (APC-Cy5.5) Mouse IgG1, K Isotype Control (Alexa Fluor® 700 Equivalent)	633	690	FC
35-4714	Phycoerythrin-Cy5.5 (PE-Cy5.5) Mouse IgG1, K Isotype Control	488	690	FC
45-4714	PerCP-Cy5.5 Mouse IgG1, K Isotype Control	488	690	FC
51-4714	Alexa Fluor® 647 Mouse IgG1, K Isotype Control	633	668	FC
53-4714	Alexa Fluor® 488 Mouse IgG1, K Isotype Control	488	519	FC
56-4714	Alexa Fluor® 700 Mouse IgG1, K Isotype Control (APC-Cy5.5 Equivalent)	633	723	FC
57-4714	Pacific Blue® Mouse IgG1, K Isotype Control	402	421	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The monoclonal mouse IgG1, κ, immunoglobulin is useful as an isotype control.

Applications Reported

For research use only, not for diagnostic or therapeutic use. APC-Alexa Fluor® 750 Mouse IgG1, K Iso Cntrl has been reported for use in flow cytometric analysis.

The photo-emission properties and compensation required for Alexa Fluor® 750 are similar to APC-Cy7; however Alexa Fluor® 750 has several advantages when compared to APC-Cy7. The Alexa Fluor® 750 has peak emission of 779 nm, and usually has a slightly higher mean fluorescent intensity when compared to the same clone conjugated with APC-Cy7, which has peak emission of 767 nm. Furthermore, Alexa Fluor® 750 is considered to be a more photostable fluorochrome as compared with APC-Cy7. It is advised not to use fixatives with Alexa Fluor® 750 as this may lead to degradation of the fluorochrome and loss of staining.

Applications Tested

APC-Alexa Fluor® 750 Mouse IgG1, K Iso Cntrl has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood. This can be used at 10 μl (0.5 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.