27-4331

Catalog Number: 27-4331
Isotype: Rat IgG2b, κ

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
16-4331	Functional Grade* Purified Rat IgG2b Isotype Control	N/A	N/A	FC
10-4331	May be discontinued; see superior product APC-Alexa Fluor® 750 Allophycocyanin-Cy7 (APC-Cy7) Rat IgG2b Isotype Control	633	760	FC
35-4331	Phycoerythrin-Cy5.5 (PE-Cy5.5) Rat IgG2b Isotype Control	488	690	FC
57-4331	DISCONTINUED - Pacific Blue® Rat IgG2b Isotype Control	402	421	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

This is a monoclonal rat IgG2b κ. It is used as an isotype control for rat IgG2b antibodies.

Applications Reported

For research use only, not for diagnostic or therapeutic use. APC-Alexa Fluor® 750 Rat IgG2b Iso Cntrl has been reported for use in flow cytometric analysis.

The photo-emission properties and compensation required for Alexa Fluor® 750 are similar to APC-Cy7; however Alexa Fluor® 750 has several advantages when compared to APC-Cy7. The Alexa Fluor® 750 has peak emission of 779 nm, and usually has a slightly higher mean fluorescent intensity when compared to the same clone conjugated with APC-Cy7, which has peak emission of 767 nm. Furthermore, Alexa Fluor® 750 is considered to be a more photostable fluorochrome as compared with APC-Cy7. It is advised not to use fixatives with Alexa Fluor® 750 as this may lead to degradation of the fluorochrome and loss of staining.

Related Products

Cat. 27-4331 Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) Rat IgG2b Isotype Control

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.