Product Information

Contents: Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) Rat IgG2b Isotype Control
Catalog Number: 27-4031
Sizes: 50 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures. This product is guaranteed for 6 months upon receipt when stored properly.
Isotype: Rat IgG2b, κ

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-4031	Fluorescein isothiocyanate (FITC) Rat IgG2b Isotype Control	488	518	FC
12-4031	Phycoerythrin (PE) Rat IgG2b Isotype Control	488	575	FC
13-4031	Biotin Rat IgG2b Isotype Control	N/A	N/A	FC
14-4031	Purified Rat IgG2b Isotype Control	N/A	N/A	FC
15-4031	Phycoerythrin-Cy5 (PE-Cy5) Rat IgG2b Isotype Control	488	670	FC
16-4031	Functional Grade* Purified Rat IgG2b Isotype Control	N/A	N/A	FC
17-4031	Allophycocyanin (APC) Rat IgG2b Isotype Control	633	660	FC
25-4031	Phycoerythrin-Cy7 (PE-Cy7) Rat IgG2b Isotype Control	488	760	FC
27-4031	Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) Rat IgG2b Isotype Control (APC-Cy7 Equivalent)	633	779	FC
36-4031	In Vivo Cytokine Capture Assay (IVCCA) In Vivo Capture Antibody – Control / Functional Grade* Biotin Rat IgG2b Isotype Control	N/A	N/A	IVCCA
45-4031	PerCP-Cy5.5 Rat IgG2b Isotype Control	488	690	FC
51-4031	Alexa Fluor® 647 Rat IgG2b Isotype Control	633	668	FC
53-4031	Alexa Fluor® 488 Rat IgG2b Isotype Control	488	519	FC
56-4031	Alexa Fluor® 700 Rat IgG2b Isotype Control	633	723	FC
57-4031	Pacific Blue® Rat IgG2b Isotype Control	402	421	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The rat IgG2b, κ monoclonal antibody is useful as an isotype control immunoglobulin.

Applications Reported

For research use only, not for diagnostic or therapeutic use. APC-Alexa Fluor® 750 Rat IgG2b Isotype Control has been reported for use in flow cytometric analysis.

The photo-emission properties and compensation required for Alexa Fluor® 750 are similar to APC-Cy7; however Alexa Fluor® 750 has several advantages when compared to APC-Cy7. The Alexa Fluor® 750 has peak emission of 779 nm, and usually has a slightly higher mean fluorescent intensity when compared to the same clone conjugated with APC-Cy7, which has peak emission of 767 nm. Furthermore, Alexa Fluor® 750 is considered to be a more photostable fluorochrome as compared with APC-Cy7. It is advised not to use fixatives with Alexa Fluor® 750 as this may lead to degradation of the fluorochrome and loss of staining.

Related Products

Cat. 27-4031 Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) Rat IgG2b Isotype Control
Cat. 27-4732 Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) Mouse IgG2b Isotype Control

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.