Contents: Allophycocyanin-Alexa FluorŽ 750 (APC-Alexa FluorŽ 750) anti-human CCR7 (CD197, BLR-2, EBI-1, CMKBR7)
Catalog Number: 27-1979
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This tandem dye is sensitive to photo-induced oxidation. Protect this vial from light during storage, handling & experimental procedures. This product is guaranteed for 6 months upon receipt when stored properly.
Clone: 3D12
Isotype: Rat IgG2a, κ
| Prices for This Product* |
| Cat. No. |
Size |
Price |
Qty |
Action |
*International customers: Please contact your distributor for region specific pricing.
|
|
 
Staining of normal human peripheral blood cells with FITC anti-human CD45RA (HI100) (cat. 11-0458) and 0.5 μg of APC-Alexa FluorŽ 750 Rat IgG2a Iso Cntrl (cat. 27-4321) (left) or APC-Alexa FluorŽ 750 anti-human CCR7 (3D12) (right). Cells in the lymphocyte gate were used for analysis.
|
| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 11-1979 |
Fluorescein isothiocyanate (FITC) anti-human CCR7 (CD197, BLR-2, EBI-1, CMKBR7) |
488 |
518 |
FC
|
| 12-1979 |
Phycoerythrin (PE) anti-human CCR7 (CD197, BLR-2, EBI-1, CMKBR7) |
488 |
575 |
FC
|
| 14-1979 |
Affinity Purified anti-human CCR7 (CD197, BLR-2, EBI-1, CMKBR7) |
N/A |
N/A |
FC
IHC(Paraffin)
|
| 27-1979 |
Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-human CCR7 (CD197, BLR-2, EBI-1, CMKBR7) |
633 |
779 |
FC
|
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The 3D12 monoclonal antibody reacts with human CCR7, also known as EBI-1 and CD197. CCR7 is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains and functions as a receptor for 6Ckine/SLC (secondary lymphoid-tissue chemokine), CCL19 and CCL21. CCR7 has been shown to be internalized via clathrin-coated pits and the majority recycled back to the plasma membrane. CCR7 is expressed on T cells and can be used to distinguish populations of naīve from central and effector memory T cells. CCR7 has been shown to play a role in migration of memory T cells to inflamed tissue. Expression of CCR7 is also found on DCs. During DC maturation CCR7 expression increases and is thought to be involved in a variety of functions: chemotaxis to the lymph node, cellular architecture, rate of endocytosis, survival and maturation. Expression of CCR7 on the cell surface can be down regulated upon ligand binding.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This 3D12 antibody has been reported for use in flow cytometric analysis.
The photo-emission properties and compensation required for Alexa FluorŽ 750 are similar to APC-Cy7; however Alexa FluorŽ 750 has several advantages when compared to APC-Cy7. The Alexa FluorŽ 750 has peak emission of 779 nm, and usually has a slightly higher mean fluorescent intensity when compared to the same clone conjugated with APC-Cy7, which has peak emission of 767 nm. Furthermore, Alexa FluorŽ 750 is considered to be a more photostable fluorochrome as compared with APC-Cy7. It is advised not to use fixatives with Alexa FluorŽ 750 as this may lead to degradation of the fluorochrome and loss of staining.
Applications Tested
This 3D12 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood cells. This can be used at 20 μl (0.5 μg) per million cells in a 100 μl total staining volume.
References
Geginat J, Lanzavecchia A, Sallusto F. Proliferation and differentiation potential of human CD8+ memory T-cell subsets in response to antigen or homeostatic cytokines.
Blood. 2003 Jun 1;101(11):4260-6. (3D12, FC,
PubMed)
Sallusto F, Lenig D, Forster R, Lipp M, Lanzavecchia A. Two subsets of memory T lymphocytes with distinct homing potentials and effector functions.
Nature. 1999 Oct 14;401(6754):708-12. (3D12, FC,
PubMed)
Related Products
Cat.
27-4321 Allophycocyanin-Alexa FluorŽ 750 (APC-Alexa FluorŽ 750) Rat IgG2a Isotype Control (APC-Cy7 Equivalent)
Alexa FluorŽ and Pacific BlueŽ are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.
Human Cellular Antigens Chart