Product Information

Contents: Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) anti-mouse CD62L (L-selectin, LECAM-1, Ly-22)
Catalog Number: 27-0621
Formulation: Phosphate buffer pH 7.2,
500 mM NaCl, 0.09% NaN₃, 0.2% BSA, 0.1% gelatin
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
This tandem dye is sensitive to photo-induced oxidation.
Protect this vial from light during storage, handling
& experimental procedures. This product is guaranteed for 6 months upon receipt when stored properly.
Clone: MEL-14
Isotype: Rat IgG2a, κ

Staining of C57Bl/6 splenocytes with APC anti-mouse CD3e (145-2C11) (cat. 17-0031) and staining buffer (autofluorescence) (left) or 0.06 μg of APC-Alexa Fluor® 750 anti-mouse CD62L (MEL-14) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0621	Affinity Purified anti-mouse CD62L (L-selectin, LECAM-1, Ly-22)	N/A	N/A	FA FC IHC IP
16-0621	Functional Grade* Purified anti-mouse CD62L (L-selectin, LECAM-1, Ly-22)	N/A	N/A	FA FC
13-0621	Biotin anti-mouse CD62L (L-selectin, LECAM-1, Ly-22)	N/A	N/A	FC
11-0621	FITC anti-mouse CD62L (L-selectin, LECAM-1, Ly-22)	488	518	FC
12-0621	PE anti-mouse CD62L (L-selectin, LECAM-1, Ly-22)	488	575	FC
15-0621	PE-Cy5 anti-mouse CD62L (L-selectin, LECAM-1, Ly-22)	488	670	FC
17-0621	APC anti-mouse CD62L (L-selectin, LECAM-1, Ly-22)	633	660	FC
25-0621	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse CD62L (L-selectin, LECAM-1, Ly-22)	488	760	FC
56-0621	Alexa Fluor® 700 anti-mouse CD62L (L-selectin, LECAM-1, Ly-22) (APC-Cy5.5 Equivalent)	633	723	FC
57-0621	Pacific Blue® anti-mouse CD62L (L-selectin, LECAM-1, Ly-22)	402	421	FC
45-0621	PerCP-Cy5.5 anti-mouse CD62L (L-selectin, LECAM-1, Ly-22) (PE-Cy5.5 replacement)	488	690	FC
93-0621	eFluor^TM 605^NC anti-mouse CD62L (L-selectin, LECAM-1, Ly-22)	350-500	605	FC
47-0621	Allophycocyanin-eFluor^TM 780 anti-mouse CD62L (L-selectin, LECAM-1, Ly-22) (APC-Alexa Fluor® 750 replacement)	633	780	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The MEL-14 monoclonal antibody reacts with mouse CD62L, a 76 kDa member of the selectin family. CD62L is expressed by neutrophils, monocytes, and subsets of T, B, and NK cells and binds a number of glycosylated, fucosylated, sulfated sialylated glycoproteins including CD34, glycam-1 and MAdCam-1. These interactions mediate rolling of lymphocytes on activated endothelium at the sites of inflammation and homing of cells to the high endothelial venules (HEV) of peripheral lymphoid tissues.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This MEL-14 antibody has been reported for use in flow cytometric analysis.

The photo-emission properties and compensation required for Alexa Fluor® 750 are similar to APC-Cy7; however Alexa Fluor® 750 has several advantages when compared to APC-Cy7. The Alexa Fluor® 750 has peak emission of 779 nm, and usually has a slightly higher mean fluorescent intensity when compared to the same clone conjugated with APC-Cy7, which has peak emission of 767 nm. Furthermore, Alexa Fluor® 750 is considered to be a more photostable fluorochrome as compared with APC-Cy7. It is advised not to use fixatives with Alexa Fluor® 750 as this may lead to degradation of the fluorochrome and loss of staining.

Applications Tested

This MEL-14 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Gallatin, W. M., I. L. Weissman, et al. (1983). "A cell-surface molecule involved in organ-specific homing of lymphocytes." Nature 304(5921): 30-4.
Siegelman, M. H., I. C. Cheng, et al. (1990). "The mouse lymph node homing receptor is identical with the lymphocyte cell surface marker Ly-22: role of the EGF domain in endothelial binding." Cell 61(4): 611-22.

Related Products

Cat. 27-4321 Allophycocyanin-Alexa Fluor® 750 (APC-Alexa Fluor® 750) Rat IgG2a Isotype Control (APC-Cy7 Equivalent)

Alexa Fluor® and Pacific Blue® are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. This product is subject to an agreement between Molecular Probes, Inc. and eBioscience, and the manufacture, use, sale or import of this product may be subject to one or more U.S. patents, pending applications and corresponding foreign equivalents, owned by Molecular Probes, Inc. (a wholly owned subsidiary of Invitrogen Corp). The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product for life science research or as an ASR. The buyer cannot use this product for manufacturing or for any other screening (specifically including use in combination with microarrays or High Content Screening) or testing purpose, other than as an ASR. For information on purchasing a license to this product for purposes other than life science research or use as an ASR, contact Molecular Probes, Inc.